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The direct and diffused components of downward shortwave radiation (SW), and photosynthetically active radiation (PAR) at the Earth surface play an essential role in biochemical (e.g. photosynthesis) and physical (e.g. energy balance) processes that control weather and climate conditions, and...
Soil microorganisms play fundamental roles in cycling of soil carbon, nitrogen, and other nutrients, yet we have a poor understanding of how soil microbiomes are shaped by their nutritional and physical environment. In this study, we investigated the successional dynamics of a soil microbiome during...
The high temporal variability of the soil-to-atmosphere CO2 flux (soil respiration, RS) has been studied at hourly to multiannual timescales, but remains less well understood than RS spatial variability. How RS fluxes vary and are auto-correlated at various time lags has practical implications for...
Soil respiration (Rs), the flow of CO2 from the soil surface to the atmosphere, is one of the largest carbon fluxes in the terrestrial biosphere. The spatial variability of Rs is both large and poorly understood, limiting our ability to robustly scale it in space. One factor in Rs spatial...
Predicting phenotypic expression from genomic and environmental information is arguably the greatest challenge in today’s biology. Being able to survey genomic content, e.g., as single-nucleotide polymorphism data, within a diverse population and predict the phenotypes of external traits, represents...
The novel fungal strain, Fusarium sp. DS 682, was isolated from the rhizosphere of the perennial grass, Bouteloua gracilis , at the Konza Prairie Biological Station in Kansas. This fungal strain is common across North American grasslands and is resilient to environmental fluctuations. The draft...
As part of the Pacific Northwest National Laboratory’s (PNNL) Science Focus Area program, we are investigating the impact of environmental change on microbial community function in grassland soils. Three grassland soils, representing different moisture regimes, were selected for ultra-deep...
The soil microbiome is central to the cycling of carbon and other nutrients and to the promotion of plant growth. Despite its importance, analysis of the soil microbiome is difficult due to its sheer complexity, with thousands of interacting species. Here, we reduced this complexity by developing...
The Community Land Model (CLM) is an effective tool to simulate the biophysical and biogeochemical processes and their interactions with the atmosphere. Although CLM Version 5 (CLM5) constitutes various updates in these processes, its performance in simulating energy, water and carbon cycles over...

Viral genome assembly annotations from terabase metagenomes (TmG.1.0) from uncultivated soil collections from the IAREC field sites in Washington (IAREC), Kansas (KBPS), and Iowa (COBS), USA. Uncultivated virus Genomes (identified from soil metagenome datasets) Data DOI Package.

Code pertaining to the Soil Microbiome SFA Project publication data visualizations 'DNA viral diversity, abundance and functional potential vary across grassland soils with a range of historical moisture regimes' for processing publication data downloads.

Data Science & Biostatistics

Dr. Paul Piehowski is the Proteomics team leader for PNNL’s Environmental and Molecular Sciences Division and the Environmental Molecular Sciences Laboratory (EMSL) user program. Piehowski is an analytical chemist whose research is focused on the application of mass spectrometry to biological...

Viral communities detected from three large grassland soil metagenomes with historically different precipitation moisture regimes.

Dataset

Complete replicate terabase metagenome (TmG.2.0) of grassland soil microbiome collections from KPBS field site in Manhattan, KS. Metagenome (unclassified soil sequencing) Data DOI Package, version 2.0.