Plot of molar ellipticity at 222 nm versus concentration of free La3+ for LanM D5E bound to lanthanum, used to calculate the LanM D5E-La3+ binding affinity (Kd).

Raw NMR HSQC spectra for LanM variants bound to La3+

Plot of molar ellipticity at 222 nm versus concentration of free Y3+ for LanM WT bound to yttrium, used to calculate the LanM WT-Y3+ binding affinity (Kd).

Plot of molar ellipticity at 222 nm versus concentration of free Y3+ for LanM D1ED5E bound to yttrium, used to calculate the LanM D1ED5E-Y3+ binding affinity (Kd).

Raw UV/Vis data measuring XO fluorescence as a function of increased [La3+]

Raw NMR HSQC spectra for LanM variants bound to Y3+

Rare earth elements (REEs) are essential to modern technologies, yet their high physical and chemical similarity makes separation of individual REEs difficult and environmentally taxing. Metalloproteins offer a promising alternative for selective REE binding, as they tend to have high metal ion...

Raw chromatographs for the HisTrap and SEC columns run to purify LanM D3ED9E.

Ribosome inactivating proteins (RIPs) such as ricin and abrin depurinate an adenine base in the sarcin/ricin loop in the large ribosomal subunit, leading to inhibtion of protein synthesis and cell death. Here, we demonstrate that RIP toxin activity can be detected via nanopore-based DNA sequencing...

This dataset consists of extreme event weather files for 115 locations in the United States, covering 16 Climate Zones in 50 states. The files were developed based on metrological data during 1995-2024 period. Severe weather events were identified during 2015-2024 and processed through a percentile...

The quantity of wastewater that is available for reuse by another party. See Tidwell et al. 2014 for details concerning how the metric was quantified. This update includes the following changes: Updated wastewater discharge and reuse data from EPA’s Clean Water Needs Assessment (see https://www.epa...

The quantity of fresh groundwater that is available for development according to the managing state or river basin authority. See Tidwell et al. 2014 for details concerning how the metric was quantified. This update includes the following changes: Utilized USGS recharge data now available at a HUC12...

Overview This dataset provides a consistent set of monthly hydropower generation for the US that reflects the local hydrologic cycle. The US Energy Information Administration (EIA) publishes monthly and annual generation data at most hydroelectric generation facilities as part of the EIA form 923...

The availability of brackish groundwater for development. See Tidwell et al. 2014 for details concerning how the metric was quantified. This update includes the following changes: Have abandoned efforts to estimate available volume as supporting information is very limited. Also believe that cost is...

The quantity of freshwater (both surface and groundwater) that could be made available by abandonment and transfer of the water right from its prior use to a new use. See Tidwell et al. 2014 for details concerning how the metric was quantified. This update includes the following changes: Simplified...