Last updated on 2023-01-30T00:09:57+00:00 by LN Anderson
MERS-CoV Experiment MCL001
The purpose of this experiment was to evaluate the human host response to wild-type Middle Eastern Respiratory Syndrome coronavirus (MERS-CoV) and mutant (icMERS-CoV-RFP, icMERS-CoV-dNSP16, icMERS-CoV-d4B, and icMERS-CoV-d3) virus infection. Sample data was obtained from human bronchial epithelial cells (Calu-3 clone 2B4) for mRNA and miRNA transcriptome expression analysis.
Secondary host-associated viral dataset downloads contain one or more statistically processed (normalization data transformation) quantitative dataset collections resulting in qualitative expression analyses of primary host-pathogen experimental study designs. Transcriptomics dataset downloads have a direct relationship to a primary sample submission corresponding to a specific MERS-CoV virus infection.
Accessible Digital Data Downloads
- Expression profiling by array (mRNA)
- Non-coding RNA profiling by array (miRNA)
Anderson, Lindsey N, Eisfeld, Amie J, Waters, Katrina M, and Modeling Host Responses to Understand Severe Human Virus Infections Program Project. Omics-Lethal Human Viruses, MERS-CoV Experiment MCL001. United States. 2021. PNNL DataHub (Web). DOI: 10.25584/LHVMCL001/1661931
Linked Primary Data Accessions
NCBI BioProject: PRJNA274404
GEO Series: GSE65574* (mRNA & miRNA transcriptome response)
*Raw measurement data has primary data publications PMID: 28830941, PMID:29152578
Acknowledgment of Federal Funding
The data described here was funded in whole or in part by the National Institute of Allergy and Infectious Diseases, of the National Institutes of Health under award number U19AI106772 and is a contribution of the "Modeling Host Responses to Understand Severe Human Virus Infections" Project at Pacific Northwest National Laboratory. Data generated by the Omics-LHV Core for proteomics, metabolomics, and lipidomics analyses for were performed at Pacific Northwest National Laboratory in the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the Department of Energy’s (DOE) Office, operating under the Battelle Memorial Institute for the DOE under contract number DE-AC05-76RLO1830.
In efforts to enable discovery, reproducibility, and reuse of NIH-funded project dataset citations, we ask that all reuse of project data and metadata download materials acknowledge all primary and secondary dataset citations where applicable and direct corresponding journal articles (Grant U19AI106772) where allowable in accordance with best practices outlined by the FORCE11 Joint Declaration of Data Citation Principles in alignment with NIH acknowledgement requirements.
CC BY-SA 4.0 (secondary dataset download DOIs), CC0 1.0 (project metadata and PNNL DataHub policy default)