Ebola Experiment EHUH001
The purpose of this experiment was to evaluate the human host response to Zaire Ebola wild-type virus and mutant virus infection. Samples were obtained from human hepatoma carcinoma cells (HUH-7) infected with Zaire Ebola ΔVP30-WT background, in addition to mutant viruses Ebola-ΔsGP (eliminating expression of soluble glycoprotein ssGP) and Ebola-Δmucin (encoding a glycoprotein lacking the mucin domain) for mRNA and miRNA transcriptome expression analysis.
Accessible Secondary Digital Data Downloads
Secondary host-associated viral dataset downloads contain one or more qualitative and/or quantitative statistically processed (normalization data transformation) expression analysis file(s) resulting from primary viral experimental study designs leveraging unique high-resolution Omics capabilities. Transcriptomic dataset downloads each have a direct relationship to a primary sample data submission corresponding to a specific Ebola virus host infection.
- Expression profiling by array (mRNA)
- Non-coding RNA profiling by array (miRNA)
Linked Primary Data Accessions
BioProject Accessions: PRJNA317724 (mRNA), PRJNA317723 (miRNA)
GEO Dataset Accessions: GSE80058 (mRNA transcriptome response), GSE80059 (miRNA transcriptome response)*
*Raw measurement data has primary data publication PMID: 34051754
Acknowledgment of Federal Funding
The data described here was funded in whole or in part by the National Institute of Allergy and Infectious Diseases, of the National Institutes of Health under award number U19AI106772 and is a contribution of the "Modeling Host Responses to Understand Severe Human Virus Infections" Project at Pacific Northwest National Laboratory. Data generated by the Omics-LHV Core for proteomics, metabolomics, and lipidomics analyses for were performed at Pacific Northwest National Laboratory in the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the Department of Energy’s (DOE) Office, operating under the Battelle Memorial Institute for the DOE under contract number DE-AC05-76RLO1830.
In efforts to enable discovery, reproducibility, and reuse of NIH-funded project dataset citations, we ask that all reuse of project data and metadata download materials acknowledge all primary and secondary dataset citations where applicable and direct corresponding journal articles (Grant U19AI106772) where allowable in accordance with best practices outlined by the FORCE11 Joint Declaration of Data Citation Principles in alignment with NIH acknowledgement requirements (NOT-OD-21-013).
CC BY-SA 4.0 (secondary dataset download DOIs), CC0 1.0 (project metadata and PNNL DataHub policy default)
Last updated on 2023-01-30T00:09:57+00:00 by LN Anderson