Omics-Lethal Human Virus, Ebola Experiment EHUH003

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Description

Ebola Experiment EHUH003

The purpose is to obtain samples for transcriptome analysis in human liver Huh cells using the following viruses obtained by reverse genetics: Zaire Ebola virus wild-type (WT) in the ΔVP30 background (wild).

Overall Design: Human liver Huh cells were infected with a Wild-type ΔVP30 Ebola virus. The Huh7 VP30 cells were on passage 5 for this experiment. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Two time courses were performed to generate samples for this experiment (the second set was initiated within 24 h of the first set). The time points used were: 0, 8, 24, 48, and 72 hours post infection (only time points 8, 24, and 48 were collected for miRNA analysis).

Processed Omics Data (available at download button):

Dataset downloads contain one or more statistically processed data file related to a lipidomic, transcriptomic, metabolomic, and/or proteomic dataset collection associated with the Ebola experimental study. 

 

Transcriptomics

mRNA

miRNA

Related Experimental Data

BioProject:  PRJNA342156PRJNA342157

GEO: GSE86539 (mRNA transcriptome response), GSE86533 (miRNA transcriptome response)

 

Acknowledgment of Federal Funding

The data described here was funded in whole or in part by the National Institute of Allergy and Infectious Diseases, of the National Institutes of Health under award number U19AI106772, as a  is a contribution of the "Modeling Host Responses to Understand Severe Human Virus Infections" Project at Pacific Northwest National Laboratory. Data generated by the Omics-LHV Core for proteomics, metabolomics, and lipidomics analyses for were performed at Pacific Northwest National Laboratory in the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the Department of Energy’s (DOE) Office, operating under the Battelle Memorial Institute for the DOE under contract number DE-AC05-76RLO1830. 

English
Projects (2)
Omics-Lethal Human Virus, Ebola
NIAID Modeling Host Responses to Understand Severe Human Virus Infections Research Program Processed Data
Publications (1)
Hypergraph models of biological networks to identify genes critical to pathogenic viral response
People (3)

Lindsey Anderson’s research has been dedicated to the identification and characterization of novel, targeted and non-targeted, functional metabolic interactions using a high-throughput systems biology and computational biology approach. Her expertise in functional metabolism and multidisciplinary...

Dr. Jason McDermott, senior research scientist, has extensive research experience in molecular and structural virology and data resource design, data integration and prediction of biological networks, bridging experimental and computational biology. Currently, his research interests include data...

Dr. Katrina Waters is the division director for Biological Sciences at the Pacific Northwest National Laboratory. Waters has a Ph.D. in biochemistry and more than 15 years of experience in microarray and proteomics data analysis. Her research interests are focused on the integration of genomics...