Ebola Experiment EHUH003
The purpose is to obtain samples for transcriptome analysis in human liver Huh cells using the following viruses obtained by reverse genetics: Zaire Ebola virus wild-type (WT) in the ΔVP30 background (wild).
Overall Design: Human liver Huh cells were infected with a Wild-type ΔVP30 Ebola virus. The Huh7 VP30 cells were on passage 5 for this experiment. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Two time courses were performed to generate samples for this experiment (the second set was initiated within 24 h of the first set). The time points used were: 0, 8, 24, 48, and 72 hours post infection (only time points 8, 24, and 48 were collected for miRNA analysis).
Processed Omics Data (available at download button):
Dataset downloads contain one or more statistically processed data file related to a lipidomic, transcriptomic, metabolomic, and/or proteomic dataset collection associated with the Ebola experimental study.
Related Experimental Data
Acknowledgment of Federal Funding
The data described here was funded in whole or in part by the National Institute of Allergy and Infectious Diseases, of the National Institutes of Health under award number U19AI106772, as a is a contribution of the "Modeling Host Responses to Understand Severe Human Virus Infections" Project at Pacific Northwest National Laboratory. Data generated by the Omics-LHV Core for proteomics, metabolomics, and lipidomics analyses for were performed at Pacific Northwest National Laboratory in the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the Department of Energy’s (DOE) Office, operating under the Battelle Memorial Institute for the DOE under contract number DE-AC05-76RLO1830.