Systems Virology Lethal Human Virus, SARS-CoV Experiment SHAE004
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The purpose of this SARS experiment was to obtain samples for transcriptome analysis in Human Airway Epithelial (HAE) cells infected with SARS-CoV, SARS deltaORF6, SARS BatSRBD mutants in a longitudinal study.
Overall Design: Primary Human airway epithelium (HAE) cells (resembling in vivo pseudo- stratified mucociliary epithelium) were infected with SARS-CoV, SARS deltaORF6, SARS BatSRBD mutant at an MOI of 2. RNA triplicates/quadruplicates are defined as 3/4 different wells, plated at the same time and using the same cell stock for all replicates. Time matched mocks done in triplicate from same cell stock as rest of samples. Culture medium (the same as what the virus stock is in) will be used for the mock infections. Time points used were: 0, 12, 24, 36, 48, 60, 72, 84, and 96 hrs post-infection.
Processed Omics Data (available at download button):
Dataset downloads contain one or more statistically processed data file related to a lipidomic, transcriptomic, metabolomic, and/or proteomic dataset collection associated with the SARS experimental study.
Related Experimental Data
GEO Dataset: GSE47962
GEO SuperSeries: GSE47963 SARS-CoV, SARS-dORF6, and SARS-BatSRBD infection of HAE cultures.
Host Factor Experiment(s): SHAE004-R (ViPR, NIAID Systems Biology)