Systems Virology Lethal Human Virus, SARS-CoV Experiment SCL006
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The purpose of this SARS experiment was to obtain samples for transcriptome and proteome analysis in human lung 2B4 cells (clonal derivative of Calu-3 cells) infected with wild type icSARS CoV Urbani and icSARS Bat SRBD (spike receptor binding domain from the wild type strain Urbani to allow for infection of human and non-human primate cells) in a longitudinal study.
Overall Design: Human lung tissue Calu-3 cells were infected with icSARS CoV and/or icSARS DORF6 mutant (MOI of 5). Each infected sample was done in triplicate at the same time using the same cell stock for all replicates, including time-matched mocks in parallel using the same cell stock as rest of samples. Time points used were: 0, 3, 7, 12, 24, 30, 36, 48, 54, 60, and 72 hrs post infection.
Processed Omics Data (available at download button):
Dataset downloads contain one or more statistically processed data file related to a lipidomic, transcriptomic, metabolomic, and/or proteomic dataset collection associated with the SARS experimental study.
Related Experimental Data
Peptide Atlas: PASS00431 (PNNL Systems Virology Contract Data)
Host Factor Experiment(s): SCL006-R, SCL006-P, SCL006 Metadata (Proteomics) (ViPR, NIAID Systems Biology)