Systems Virology Lethal Human Virus, SARS-CoV Experiment SCL006

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Systems Virology Lethal Human Virus, SARS-CoV Experiment SCL006

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The purpose of this SARS experiment was to obtain samples for transcriptome and proteome analysis in human lung 2B4 cells (clonal derivative of Calu-3 cells) infected with wild type icSARS CoV Urbani and icSARS Bat SRBD (spike receptor binding domain from the wild type strain Urbani to allow for infection of human and non-human primate cells) in a longitudinal study. 

Overall Design: Human lung tissue Calu-3 cells were infected with icSARS CoV and/or icSARS DORF6 mutant (MOI of 5). Each infected sample was done in triplicate at the same time using the same cell stock for all replicates, including time-matched mocks in parallel using the same cell stock as rest of samples. Time points used were: 0, 3, 7, 12, 24, 30, 36, 48, 54, 60, and 72 hrs post infection.

Processed Omics Data (available at download button):

Dataset downloads contain one or more statistically processed data file related to a lipidomic, transcriptomic, metabolomic, and/or proteomic dataset collection associated with the SARS experimental study.





Related Experimental Data

BioProject:  PRJNA163617

GEO:  GSE37827

Peptide Atlas:  PASS00431 (PNNL Systems Virology Contract Data)

Host Factor Experiment(s):  SCL006-RSCL006-PSCL006 Metadata (Proteomics) (ViPR, NIAID Systems Biology)

DOI: 10.1073/pnas.0808116105

Publications (2)
People (3)

Lindsey Anderson’s research has been dedicated to the identification and characterization of novel, targeted and non-targeted, functional metabolic interactions using a high-throughput systems biology and computational biology approach. Her expertise in functional metabolism and multidisciplinary...

Dr. Jason McDermott, senior research scientist, has extensive research experience in molecular and structural virology and data resource design, data integration and prediction of biological networks, bridging experimental and computational biology. Currently, his research interests include data...

Dr. Katrina Waters is the division director for Biological Sciences at the Pacific Northwest National Laboratory. Waters has a Ph.D. in biochemistry and more than 15 years of experience in microarray and proteomics data analysis. Her research interests are focused on the integration of genomics...