Systems Virology Lethal Human Virus, SARS-CoV Experiment SCL008
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The purpose of this SARS experiment was to obtain samples for transcriptome and proteome analysis in human lung 2B4 cells (clonal derivative of Calu-3 cells) infected with either icSARS CoV, icSARS-deltaNSP16 or icSARS ExoNI mutants in a longitudinal study.
Overall Design: Human lung tissue 2B4 cells (clonal derivatives of Calu-3 cells) were infected with either icSARS CoV, icSARS deltaNSP16 or icSARS ExoNI (wild type and deltaNSP16 mutant infections were at an MOI of 5 while the ExoNI infections were at an MOI of 1). Each infected sample was done in triplicate (triplicates are defined as 3 different wells at the same time using the same cell stock for all replicates), including time-matched mocks in parallel using the same cell stock as rest of samples. Time points used were: 0, 7, 12, 24, 30, 36, 48, 60, and 72 hrs post infection.
Processed Omics Data (available at download button):
Dataset downloads contain one or more statistically processed data file related to a lipidomic, transcriptomic, metabolomic, and/or proteomic dataset collection associated with the SARS experimental study.
Related Experimental Data
Peptide Atlas: PASS00432 (PNNL Systems Virology Contract Data)
Host Factor Experiment(s): SCL008-R, SCL008-P, SCL008 Metadata (Proteomics) (ViPR, NIAID Systems Biology)