Omics-LHV, West Nile
The purpose of this West Nile experiment was to obtain samples for omics analysis in primary mouse granule neuron cells infected with wild type West Nile virus (WNV-NY99 clone 382, WNVWT) and mutant virus (WNVE218A).
Overall Design: Granule cell neurons from day 6 C57Bl/6J mouse pups were infected with plasmid-derived wild type West Nile virus NY99 clone 382 (WNV-NY99 clone 382, WNVWT) or plasmid-derived West Nile virus NY99 clone 382 isogenic E218A mutant (WNVE218A) virus. Infection were done in triplicate with a multiplicity of infection (MOI) of 250. Time matched mocks were collected in parallel using cell media concentrated through ultracentrifugation and diluted as virus. Proteomic samples were pooled for mutant WNV and mockulum.
Processed Omics Data (available at download button):
Dataset downloads contain one or more statistically processed data file related to a lipidomic, transcriptomic, metabolomic, and/or proteomic dataset collection associated with a West Nile experimental study.
Related Experimental Data
PRIDE: PXD003301 (proteome response)
MassIVE: MSV000079415 (proteome response)