Omics-Lethal Human Viruses, West Nile Experiment WGCN004

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Omics-LHV, West Nile

Experiment WGCN004


The purpose of this West Nile experiment was to obtain samples for omics analysis in primary mouse granule neuron cells infected with wild type West Nile virus (WNV-NY99 clone 382, WNVWT) and mutant virus (WNVE218A).

Overall Design: Granule cell neurons from day 6 C57Bl/6J mouse pups were infected with plasmid-derived wild type West Nile virus NY99 clone 382 (WNV-NY99 clone 382, WNVWT) or plasmid-derived West Nile virus NY99 clone 382 isogenic E218A mutant (WNVE218A) virus. Infection were done in triplicate with a multiplicity of infection (MOI) of 250. Time matched mocks were collected in parallel using cell media concentrated through ultracentrifugation and diluted as virus. Proteomic samples were pooled for mutant WNV and mockulum. 

Processed Omics Data (available at download button):

Dataset downloads contain one or more statistically processed data file related to a lipidomic, transcriptomic, metabolomic, and/or proteomic dataset collection associated with a West Nile experimental study. 





Related Experimental Data

PRIDE: PXD003301 (proteome response)

MassIVE: MSV000079415 (proteome response)

People (3)

Lindsey Anderson’s research has been dedicated to the identification and characterization of novel, targeted and non-targeted, functional metabolic interactions using a high-throughput systems biology and computational biology approach. Her expertise in functional metabolism and multidisciplinary...

Dr. Jason McDermott, senior research scientist, has extensive research experience in molecular and structural virology and data resource design, data integration and prediction of biological networks, bridging experimental and computational biology. Currently, his research interests include data...

Dr. Katrina Waters is the division director for Biological Sciences at the Pacific Northwest National Laboratory. Waters has a Ph.D. in biochemistry and more than 15 years of experience in microarray and proteomics data analysis. Her research interests are focused on the integration of genomics...