Publication - Journal Article Current Production and Metal Oxide Reduction by Shewanella oneidensis MR-1 Wild Type and Mutants

Applied and Environmental Microbiology, vol. 73, iss. 21, pp. 7003-7012, 2007

Orianna Bretschger, Anna Obraztsova, Carter A. Sturm, In Seop Chang, Yuri A. Gorby, Samantha B. Reed, David E. Culley, Catherine L. Reardon, Soumitra Barua, Margaret F. Romine, Jizhong Zhou, Alexander S. Beliaev, Rachida Bouhenni, Daad Saffarini, Florian Mansfeld, Byung-Hong Kim, James K. Fredrickson, Kenneth H. Nealson

ABSTRACT Shewanella oneidensis MR-1 is a gram-negative facultative anaerobe capable of utilizing a broad range of electron acceptors, including several solid substrates. S. oneidensis MR-1 can reduce Mn(IV) and Fe(III) oxides and can produce current in microbial fuel cells. The mechanisms that are employed by S. oneidensis MR-1 to execute these processes have not yet been fully elucidated. Several different S. oneidensis MR-1 deletion mutants were generated and tested for current production and metal oxide reduction. The results showed that a few key cytochromes play a role in all of the processes but that their degrees of participation in each process are very different. Overall, these data suggest a very complex picture of electron transfer to solid and soluble substrates by S. oneidensis MR-1.