Journal Article
mSphere, vol. 1, iss. 2, 2016
Authors
Ludmila Matos Baltazar, Ernesto S. Nakayasu, Tiago J. P. Sobreira, Hyungwon Choi, Arturo Casadevall, Leonardo Nimrichter, Joshua D. Nosanchuk, J. Andrew Alspaugh
Abstract
Diverse fungal species release extracellular vesicles, indicating that this is a common pathway for the delivery of molecules to the extracellular space. However, there has been no study reporting the impact of antibody binding to the fungal cell on extracellular vesicle release. In the present work, we observed that treatment of
H. capsulatum
cells with Hsp60-binding MAbs significantly changed the size and cargo of extracellular vesicles, as well as the enzymatic activity of certain virulence factors, such as laccase and phosphatase. Furthermore, this finding demonstrates that antibody binding can directly impact protein loading in vesicles and fungal metabolism. Hence, this work presents a new role for antibodies in the modification of fungal physiology.
