Publication - Journal Article Multiomics resolution of molecular events during a day in the life of Chlamydomonas

Proceedings of the National Academy of Sciences, vol. 116, iss. 6, pp. 2374-2383, 2019

Daniela Strenkert, Stefan Schmollinger, Sean D. Gallaher, Patrice A. Salomé, Samuel O. Purvine, Carrie D. Nicora, Tabea Mettler-Altmann, Eric Soubeyrand, Andreas P. M. Weber, Mary S. Lipton, Gilles J. Basset, Sabeeha S. Merchant

Significance Chlamydomonas reinhardtii is the premier reference organism for understanding unicellular green algae. Chlamydomonas is an important model for photosynthesis as well as fermentation and other anaerobic pathways under dark anoxic conditions. We have produced a diurnal transcriptome, validated by subproteomic analyses, and matched with measurements of pigments, select metabolites, and physiological parameters. We report that the majority of the algal genome is differentially expressed over the course of the day and the timing of specific genes is dictated by their biological function. We also discovered that fermentation rather than respiration is the preferred metabolic fate of starch-derived glycolytic pyruvate. We offer our rich dataset to the algal and plant communities.