Publication - Journal Article MetFish: a Metabolomics Pipeline for Studying Microbial Communities in Chemically Extreme Environments

https://doi.org/10.1128/msystems.01058-20
Journal Article
mSystems, vol. 6, iss. 3, 2021
Authors
Chengdong Xu, Sneha P. Couvillion, Ryan L. Sontag, Nancy G. Isern, Yukari Maezato, Stephen R. Lindemann, Taniya Roy Chowdhury, Rui Zhao, Beau R. Morton, Rosalie K. Chu, Ronald J. Moore, Janet K. Jansson, Vanessa L. Bailey, Paula J. Mouser, Margaret F. Romine, James F. Fredrickson, Thomas O. Metz, Marcelino Gutiérrez
Abstract
The identification and accurate quantification of metabolites using electrospray ionization-mass spectrometry (ESI-MS) in hypersaline samples is a challenge due to matrix effects. Clean-up and desalting strategies that typically work well for samples with lower salt concentrations are often ineffective in hypersaline samples.
English