Data Source - Instrument Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer

Created on 2025-02-03T23:25:06+00:00 by LN Anderson

The Thermo Scientific™ Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer data source system combines a high-resolution, accurate-mass (HRAM) ultra-high-field Orbitrap mass analyzer, high capacity transfer tube with electrodynamic ion funnel, and high performance quadrupole precursor ion selection to deliver unsurpassed speed and sensitivity. The Q Exactive HF-X enables discovery and targeted quantification of proteins using various label-free, SILAC, or isotopically labeled tandem mass tags (TMT) quantitation strategies with parallel reaction monitoring validation. With combined HRAM capabilities and a high-resolution data-independent acquisition (DIA) workflow, excellent peptide quantitation with high reproducibility can be achieved.

Data Source Contributions

• LN Anderson, Data Curation
• RJ Moore, Resource Review

Instrument Data Source Profile

Instrument Name:      Q Exactive HF-X

Model:                         Thermo Scientific™ Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer

Platform Design:        LC-HMS with MS/MS; optional HCD capabilities

Data Types:                 DIA-HMS-HCD-HMSn, HMS, HMS-HCD-HMSn, HMS-HMSn

Instrument Group:      Liquid Chromatography Mass Spectrometry

Topic Areas:                Mass Spectrometry Proteomics, Bottom-Up Proteomics

Explore this capability and more at the Environmental Molecular Sciences Laboratory (EMSL) Bottom-Up (fragmented) Proteomics instrument resource page. For a list of complimentary mass spectrometry data process software and analytical workflows, visit https://zenodo.org/communities/pnnl. 

Federal Acknowledgements

This work was supported in part by the Predictive Phenomics Initiative (PPI) at Pacific Northwest National Laboratory (PNNL), a multiprogram national laboratory managed by the Battelle Memorial Institute, operating under the U.S. Department of Energy (DOE) contract DE-AC05-76RL01830. User capabilities described here reflect collaborations with the Environmental Molecular Sciences Laboratory (EMSL), a DOE Office of Science (SC-3) user facility operating under the Contract No. DE-AC05-76RL01830.

Terms of Use

Recommendation guidelines provided by the DOE Office of Science can be accessed at the SC Funding Opportunities & Acknowledgements homepage. For additional information regarding user capability data release, visit the SC Digital Data Management Resources at User Facilities for more information.

EMSL Funding Acknowledgment

Scientists who publish results of research using EMSL resources, capabilities, and resulting data are required to include an acknowledgment of EMSL Policies in any publications. Learn more about user facility data management resources at the Office of Science Program page.