Omics-Lethal Human Virus, Interferon Experiment IFNαIHH001
The purpose of this experiment was to evaluate the human host cell response to human interferon α (IFNα) treatment. Sample data was obtained from human IHH (immortalized human hepatocyte) cells for mRNA and miRNA transcriptome data analysis.
Overall Design: Immortalized human hepatocyte (IHH) cells were seeded at 1 x 10^6 cells per well on 6-well plates 3 days prior to infection. Treatments were initiated by replacing culture medium (containing 10% FBS) with fresh medium containing 500 U/ml of human interferon α (PBL Biosciences). Mock-treated cells were given normal culture medium only. Mock and treated cells were incubated at 37°C. Infected samples were collected in triplet; time-matched mocks were collected in triplet in parallel with infected samples. Transcriptome sample collection time points: 6, 12, and 18 hrs post-infection.
- Human Interferon α (IFNα) treatment (500 U/ml)
Processed Omics Data (available at download button):
Dataset downloads contain one or more statistically processed data file related to a lipidomic, transcriptomic, metabolomic, and/or proteomic dataset collection associated with an Interferon-stimulated response experimental study.
Expression profiling by array (mRNA)
Non-coding RNA profiling by array (miRNA)
Related Experimental Data
GEO SuperSeries: GSE65575 (Modeling Host Responses to Understand Severe Human Virus Infections)