OmicsLHV-WCN002

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Description

Omics-LHV, West Nile

Experiment WCN002

 

The purpose of this West Nile experiment was to obtain samples for transcriptome analysis in mouse cortical neuron collections infected with wild type West Nile virus (WNV NY99 clone 382; WNVWT) and mutant West Nile virus (WNVE218A). This data set comprises two complete biological replicate experiments conducted in the same conditions and with data processed independently (WCN002 and WCN003).

Overall Design: Primary cortical neurons from C57Bl/6J mouse embryos (age E15) were infected with plasmid-derived wild type West Nile virus NY99 clone 382 (WNVWT) or plasmid-derived isogenic E218A mutant West Nile virus NY99 clone 382 (WNVE218A) in multiplicity of infection (MOI) of 250 in triplicate. Time matched mocks were collected in parallel using cell media concentrated through ultracentrifugation and diluted as virus. Time points used were: 1, 8, 12, and 24 hrs post infection (miRNA: 12 hrs only).

See Experiment WCN003 for corresponding independent biological replicate study. 

Processed Omics Data (available at download button):

Dataset downloads contain one or more statistically processed data file related to a lipidomic, transcriptomic, metabolomic, and/or proteomic dataset collection associated with a West Nile experimental study. 

 

Transcriptomics

mRNA

miRNA

Related Experimental Data

BioProject:  PRJNA280008PRJNA280010

GEO: GSE67473 (mRNA transcriptome response), GSE67474 (miRNA transcriptome response)

DOI: 10.1038/nm.3108 (GEO: GSE42727)

English
Projects (2)
Omics-Lethal Human Virus, West Nile
NIAID Modeling Host Responses to Understand Severe Human Virus Infections Research Program Processed Data
People (3)

Lindsey Anderson’s research has been dedicated to the identification and characterization of novel, targeted and non-targeted, functional metabolic interactions using a high-throughput systems biology and computational biology approach. Her expertise in functional metabolism and multidisciplinary...

Dr. Jason McDermott, senior research scientist, has extensive research experience in molecular and structural virology and data resource design, data integration and prediction of biological networks, bridging experimental and computational biology. Currently, his research interests include data...

Dr. Katrina Waters is the division director for Biological Sciences at the Pacific Northwest National Laboratory. Waters has a Ph.D. in biochemistry and more than 15 years of experience in microarray and proteomics data analysis. Her research interests are focused on the integration of genomics...