Omics-LHV, West Nile
The purpose of this West Nile experiment was to obtain samples for transcriptome analysis in primary mouse myeloid dendritic cells infected with wild type West Nile virus (WNVMT) and mutant virus (WNVE218A).
Overall Design: Mouse (C57Bl/6J) primary amyeloid dendritic cells (D10 after seeding of leukocytes from bone marrow) were seeded at 2 x 10^5 cells per well of non-tc treated 12-well plates in 0.8 mL R10 DC media after infection at a multiplicity of infection (MOI) of 500. Infected samples were then collected in quintuplet in addition to time-matched mocks in parallel with infected samples. Time points used were: 1, 8, 12, and 24 hrs post-infection.
See Experiment WDC010 (GSE74628) for corresponding independent biological replicate study.
Processed Omics Data (available at download button):
Dataset downloads contain one or more statistically processed data file related to a lipidomic, transcriptomic, metabolomic, and/or proteomic dataset collection associated with a West Nile experimental study.
Related Experimental Data
GEO: GSE75222 (mRNA transcriptome response)