Omics-Lethal Human Viruses, West Nile Experiment WDC011

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Description

Omics-LHV, West Nile

Experiment WDC011

 

The purpose of this West Nile experiment was to obtain samples for transcriptome analysis in primary mouse myeloid dendritic cells infected with wild type West Nile virus (WNVMT) and mutant virus (WNVE218A).

Overall Design: Mouse (C57Bl/6J) primary amyeloid dendritic cells (D10 after seeding of leukocytes from bone marrow) were seeded at 2 x 10^5 cells per well of non-tc treated 12-well plates in 0.8 mL R10 DC media after infection at a multiplicity of infection (MOI) of 500. Infected samples were then collected in quintuplet in addition to time-matched mocks in parallel with infected samples. Time points used were: 1, 8, 12, and 24 hrs post-infection.

See Experiment WDC010 (GSE74628) for corresponding independent biological replicate study.

Processed Omics Data (available at download button):

Dataset downloads contain one or more statistically processed data file related to a lipidomic, transcriptomic, metabolomic, and/or proteomic dataset collection associated with a West Nile experimental study. 

 

Transcriptomics

mRNA

Related Experimental Data

BioProject:  PRJNA302822

GEO:  GSE75222 (mRNA transcriptome response)

English
Projects (2)
Omics Lethal Human Viruses Project Profiling of the Host Response to West Nile Virus Infection, Processed Experimental Dataset Catalog
NIAID Modeling Host Responses to Understand Severe Human Virus Infections, Multi-Omic Viral Dataset Catalog Collections
People (3)

Lindsey Anderson’s research has been dedicated to the identification and characterization of novel, targeted and non-targeted, functional metabolic interactions using a high-throughput systems biology and computational biology approach. Her expertise in functional metabolism and multidisciplinary...

Dr. Jason McDermott, senior research scientist, has extensive research experience in molecular and structural virology and data resource design, data integration and prediction of biological networks, bridging experimental and computational biology. Currently, his research interests include data...

Dr. Katrina Waters is the division director for Biological Sciences at the Pacific Northwest National Laboratory. Waters has a Ph.D. in biochemistry and more than 15 years of experience in microarray and proteomics data analysis. Her research interests are focused on the integration of genomics...