OmicsLHV-WDC010

Dataset Image

download Download

Description

Omics-LHV, West Nile

Experiment WDC010

 

The purpose of this West Nile experiment was to obtain samples for omics analysis in primary mouse myeloid dendritic cells infected with wild type West Nile virus (WNVMT) and mutant virus (WNVE218A).

Overall Design: Mouse (C57Bl/6J) primary amyeloid dendritic cells (D10 after seeding of leukocytes from bone marrow) were seeded at 2 x 10^5 cells per well of non-tc treated 12-well plates in 0.8 mL R10 DC media after infection at a multiplicity of infection (MOI) of 500. Infected samples were then collected in quintuplet in addition to time-matched mocks in parallel with infected samples. Time points used were: 1, 8, 12, and 24 hrs post-infection.

See Experiment WDC011 (GSE75222) for corresponding independent biological replicate study.

Processed Omics Data (available at download button):

Dataset downloads contain one or more statistically processed data file related to a lipidomic, transcriptomic, metabolomic, and/or proteomic dataset collection associated with a West Nile experimental study. 

 

Transcriptomics

mRNA

Proteomics

Related Experimental Data

BioProject:  PRJNA301036

GEO:  GSE74628 (mRNA transcriptome response)

PRIDE: PXD003507 (proteome response)

MassIVE:  MSV000079470 (proteome response)

English
Projects (2)
Omics-Lethal Human Virus, West Nile
NIAID Modeling Host Responses to Understand Severe Human Virus Infections Research Program Processed Data
People (3)

Lindsey Anderson’s research has been dedicated to the identification and characterization of novel, targeted and non-targeted, functional metabolic interactions using a high-throughput systems biology and computational biology approach. Her expertise in functional metabolism and multidisciplinary...

Dr. Jason McDermott, senior research scientist, has extensive research experience in molecular and structural virology and data resource design, data integration and prediction of biological networks, bridging experimental and computational biology. Currently, his research interests include data...

Dr. Katrina Waters is the division director for Biological Sciences at the Pacific Northwest National Laboratory. Waters has a Ph.D. in biochemistry and more than 15 years of experience in microarray and proteomics data analysis. Her research interests are focused on the integration of genomics...