Velos Pro Orbitrap Mass Spectrometer

Description

The Thermo Scientific (https://www.thermofisher.com/) "Velos Pro Orbitrap Mass Spectrometer" [MS:1003096] instrument data source combines advanced mass accuracy with an ultra-high resolution Orbitrap mass analyzer for increased sensitivity, enabling molecular weight determination for intact proteins and in-depth analyses of isobaric species. The Velos Pro Orbitrap instrument additionally provides high resolution mass spectra (HMS) with optional electron transfer dissociation (ETD) [MS:1000598] and higher-energy collisional dissociation (HCD) [MS:1002481] for generating complementary information supporting highly sensitive post-translational modification (PTM) analytical results and de novo sequencing. Lipid analyses are performed by coupling an MS inlet to an Agilent/Waters system and a mobile phase optimized for lipid soluble metabolites.

Explore this capability and more at EMSL's Omics & Mass Spectrometer (MS) resource page under Omics and Mass Spectrometry for Lipidomics. For a list of complimentary mass spectrometry data process software and analytical workflows, visit https://zenodo.org/communities/pnnl

Data Source Contributions

Instrument Data Source Profile

Instrument Name:     Velos Pro Orbitrap Mass Spectrometer [MS:1003096]; VelosOrbiETD

Model:                         Thermo Scientific LTQ Velos MS with ETD [MS:1000856]

Platform Design:        Velos Pro Orbitrap (LC-HMS with MS/MS, equipped with ETD/HCD capabilities)

Acquisition Data:        HMS-HCD-CID-MSn

Method Taxon:            Mass spectrometry > Liquid Chromatography-Mass Spectrometry > High Resolution ESI Mass Spectrometry,

                                      High-Energy Collision-Induced Dissociation (HCD/CID),

                                      Reversed-Phase Liquid Chromatography-Electrospray Ionisation Mass Spectrometry (RPLC-LC-ESI-MS/MS)

Topic Areas:                 Omics > Proteomics, Lipidomics

 

Acknowledgements

This work was supported in part by the Earth and Biological Sciences Directorate (EBSD) at Pacific Northwest National Laboratory (PNNL), a multiprogram national laboratory managed by the Battelle Memorial Institute, operating under the U.S. Department of Energy (DOE) contract DE-AC05-76RL01830. User capabilities described here reflect collaborations with the Environmental Molecular Sciences Laboratory (EMSL), a DOE Office of Science (SC-3) user facility operating under the Contract No. DE-AC05-76RL01830.

Terms of Use

Scientists who publish results of research using EMSL resources, capabilities, and resulting data are required to include an acknowledgment of EMSL Policies in any publications. Learn more about user facility data management resources at the Office of Science Program page.

     

    Projects (8)

    The Phenotypic Response of the Soil Microbiome to Environmental Perturbations Project (Soil Microbiome SFA) at Pacific Northwest National Laboratory is a Genomic Sciences Program Science Focus Area (SFA) Project operating under the Environmental Microbiome Science Research Area. The Soil Microbiome...

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    Omics-LHV Profiling of Host Response to Influenza A Virus Infection Background: Influenza A viruses (IAV) are classified as a Category C priority pathogen, by the National Institute of Allergy and Infectious Diseases (NIAID), and are known to cause severe and even fatal infections in humans where...

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    Omics-LHV Profiling of Host Response to Ebola Virus Infection Background Ebola virus (EBOV) is high risk biological agent classified as a Category A priority pathogen by the National Institute of Allergy and Infectious Diseases (NIAID) and is known to cause severe respiratory disease or hemorrhagic...

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    Omics-LHV Profiling of Host Response to MERS-CoV Virus Infection Background: Middle East Respiratory Syndrome coronavirus (MERS-CoV) is classified as a Category C priority pathogen, by the National Institute of Allergy and Infectious Diseases (NIAID), and are known to cause severe and even fatal...

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    Omics-LHV Systems Biology Modeling Host Response to Severe Acute Respiratory Syndrome (SARS) Infection The Systems Virology project was one of four systems biology contracts funded by the NIAID Systems Biology for Infectious Diseases Research Program from 2008-2013. The Systems Biology for...

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    Omics-LHV Profiling of Host Response to West Nile Virus Infection Background: West Nile virus (WNV) is classified as a Category B priority pathogen, by the National Institute of Allergy and Infectious Diseases (NIAID), and are known to cause severe and even fatal infections in humans where lethal...

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    Reusable Digital Data Lifecycle Downloads for Modeling Host Responses to Understand Severe Human Virus Infections The National Institute of Allergy and Infectious Diseases (NIAID) "Modeling Host Responses to Understand Severe Human Virus Infections" (U19AI106772) program project was a highly...

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    PerCon SFA Project Publication Linked Data Catalog (uploads pending) The Persistence Control of Engineered Functions in Complex Soil Microbiomes Project (PerCon SFA), at Pacific Northwest National Laboratory (PNNL) is a Genomic Sciences Program, Biosystems Design, Research Science Focus Area project...

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    Lindsey Anderson’s research has been dedicated to the identification and characterization of novel, targeted and non-targeted, functional metabolic interactions using a high-throughput systems biology and computational biology approach. Her expertise in functional metabolism and multidisciplinary...

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