Velos Pro Orbitrap Mass Spectrometer

Description

The Thermo Scientific LTQ Orbitrap Velos combines the proven mass accuracy and ultra-high resolution of the Orbitrap mass analyzer, with the increased sensitivity and improved cycle time of the LTQ Velos. The high mass accuracy of the LTQ Orbitrap Velos increases the speed and condense of indication in complex samples by minimizing false positive identification. The ultra-high resolution provides certainty in analytical results by enabling molecular weight determination for intact proteins and in-depth analysis of isobaric species. The Velos Pro LTQ-Orbitrap provides high resolution mass spectrometer data with optional HCD, or ETD capability. Electron Transfer Dissociation (ETD) generates complementary information for highly sensitive PTM analyses and de novo sequencing. Lipid analyses are performed by coupling an MS inlet to an Agilent/Waters system and a mobile phase optimized for lipid soluble metabolites.

Data Source Contributions

Instrument Data Source Profile

Instrument Name:     Velos Pro Orbitrap Mass Spectrometer

Model:                         Thermo Scientific LTQ Orbitrap Velos Pro 

Platform:                     Velos Pro Orbitrap (LC-HMS with MS/MS, equipped with ETD and optional HCD capability)

Data Acquisition:        HMS-HCD-CID-MSn

Method Taxon:            Mass spectrometryLiquid Chromatography-Mass Spectrometry, High Resolution ESI Mass Spectrometry,

                                      High-Energy Collision-Induced Dissociation (HCD/CID)

                                      Reversed-Phase Liquid Chromatography-Electrospray Ionisation Mass Spectrometry (RPLC-LC-ESI-MS/MS)

Topic Areas:                 OmicsProteomics, Lipidomics

 

Explore this capability and more at EMSL's Omics & Mass Spectrometer (MS) resource page under Omics and Mass Spectrometry for Lipidomics. For a list of complimentary mass spectrometry data process software and analytical workflows, visit https://zenodo.org/communities/pnnl

 

Acknowledgements

This work was supported in part by the Earth and Biological Sciences Directorate (EBSD) at Pacific Northwest National Laboratory (PNNL), a multiprogram national laboratory managed by the Battelle Memorial Institute, operating under the U.S. Department of Energy (DOE) contract DE-AC05-76RL01830. User capabilities described here reflect collaborations with the Environmental Molecular Sciences Laboratory (EMSL), a DOE Office of Science (SC-3) user facility operating under the Contract No. DE-AC05-76RL01830.

Terms of Use

Scientists who publish results of research using EMSL resources, capabilities, and resulting data are required to include an acknowledgment of EMSL Policies in any publications. Learn more about user facility data management resources at the Office of Science Program page.

Projects (8)

The Phenotypic Response of the Soil Microbiome to Environmental Perturbations Project (Soil Microbiome SFA) at Pacific Northwest National Laboratory is a Genomic Sciences Program Science Focus Area (SFA) Project operating under the Environmental Microbiome Science Research Area. The Soil Microbiome...

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    13

Omics-LHV Profiling of Host Response to Influenza A Virus Infection Background: Influenza A viruses (IAV) are classified as a Category C priority pathogen, by the National Institute of Allergy and Infectious Diseases (NIAID), and are known to cause severe and even fatal infections in humans where...

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    9

Omics-LHV Profiling of Host Response to Ebola Virus Infection Background: Ebola virus (EBOV) is classified as a Category A priority pathogen, by the National Institute of Allergy and Infectious Diseases (NIAID), and are known to cause severe and even fatal infections in humans where lethal host...

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    8

Omics-LHV Profiling of Host Response to MERS-CoV Virus Infection Background: Middle East Respiratory Syndrome coronavirus (MERS-CoV) is classified as a Category C priority pathogen, by the National Institute of Allergy and Infectious Diseases (NIAID), and are known to cause severe and even fatal...

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    14

Omics-LHV Systems Biology Modeling Host Response to Severe Acute Respiratory Syndrome (SARS) Infection The Systems Virology project was one of four systems biology contracts funded by the NIAID Systems Biology for Infectious Diseases Research Program from 2008-2013. The Systems Biology for...

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    11

Omics-LHV Profiling of Host Response to West Nile Virus Infection Background: West Nile virus (WNV) is classified as a Category B priority pathogen, by the National Institute of Allergy and Infectious Diseases (NIAID), and are known to cause severe and even fatal infections in humans where lethal...

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    14

Reusable Digital Data Lifecycle Downloads for Modeling Host Responses to Understand Severe Human Virus Infections The National Institute of Allergy and Infectious Diseases (NIAID) "Modeling Host Responses to Understand Severe Human Virus Infections" (U19AI106772) program project was a highly...

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    50

PerCon SFA Project Publication Linked Data Catalog (uploads pending) The Persistence Control of Engineered Functions in Complex Soil Microbiomes Project (PerCon SFA), at Pacific Northwest National Laboratory (PNNL) is a Genomic Sciences Program, Biosystems Design, Research Science Focus Area project...

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Lindsey Anderson’s research has been dedicated to the identification and characterization of novel, targeted and non-targeted, functional metabolic interactions using a high-throughput systems biology and computational biology approach. Her expertise in functional metabolism and multidisciplinary...

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