Data Source - Mass Spectroscopy (MS) Velos Pro Orbitrap Mass Spectrometer

The Thermo Scientific (https://www.thermofisher.com/) "Velos Pro Orbitrap Mass Spectrometer" [MS:1003096] instrument data source combines advanced mass accuracy with an ultra-high resolution Orbitrap mass analyzer for increased sensitivity, enabling molecular weight determination for intact proteins and in-depth analyses of isobaric species. The Velos Pro Orbitrap instrument additionally provides high resolution mass spectra (HMS) with optional electron transfer dissociation (ETD) [MS:1000598] and higher-energy collisional dissociation (HCD) [MS:1002481] for generating complementary information supporting highly sensitive post-translational modification (PTM) analytical results and de novo sequencing. Lipid analyses are performed by coupling an MS inlet to an Agilent/Waters system and a mobile phase optimized for lipid soluble metabolites.

Data Source Contributions

• LN Anderson > Data Curation
• RJ Moore > Resource Review

Instrument Data Source Profile

Instrument Name:     Velos Pro Orbitrap Mass Spectrometer [MS:1003096]; VelosOrbiETD

Model:                         Thermo Scientific LTQ Velos MS with ETD [MS:1000856]

Platform Design:        Velos Pro Orbitrap (LC-HMS with MS/MS, equipped with ETD/HCD capabilities)

Acquisition Data:        HMS-HCD-CID-MSn

Method Taxon:            Mass spectrometry > Liquid Chromatography-Mass Spectrometry > High Resolution ESI Mass Spectrometry,

                                      High-Energy Collision-Induced Dissociation (HCD/CID),

                                      Reversed-Phase Liquid Chromatography-Electrospray Ionisation Mass Spectrometry (RPLC-LC-ESI-MS/MS)

Topic Areas:                 Omics > Proteomics, Lipidomics

Explore this capability and more at EMSL's Omics & Mass Spectrometer (MS) resource page under Omics and Mass Spectrometry for Lipidomics. For a list of complimentary mass spectrometry data process software and analytical workflows, visit https://zenodo.org/communities/pnnl. 

Federal Acknowledgements

This work was supported in part by the Earth and Biological Sciences Directorate (EBSD) at Pacific Northwest National Laboratory (PNNL), a multiprogram national laboratory managed by the Battelle Memorial Institute, operating under the U.S. Department of Energy (DOE) contract DE-AC05-76RL01830. User capabilities described here reflect collaborations with the Environmental Molecular Sciences Laboratory (EMSL), a DOE Office of Science (SC-3) user facility operating under the Contract No. DE-AC05-76RL01830.

Terms of Use

Recommendation guidelines provided by the DOE Office of Science can be accessed at the SC Funding Opportunities & Acknowledgements homepage. For additional information regarding user capability data release, visit the SC Digital Data Management Resources at User Facilities for more information.

EMSL Funding Acknowledgment

Scientists who publish results of research using EMSL resources, capabilities, and resulting data are required to include an acknowledgment of EMSL Policies in any publications. Learn more about user facility data management resources at the Office of Science Program page.