Dataset - Omics, Proteomics, Transcriptomics, Metabolomics, Lipidomics Omics-Lethal Human Viruses, Influenza A Experiment ICL103

Last updated on 2024-02-11T22:41:43+00:00 by LN Anderson

Influenza A Virus Experiment ICL103

The purpose of this experiment was to evaluate the human host response to Influenza A virus (subtype H5N1) and mutant viruses. Sample data was obtained for human lung adenocarcinoma cell line (Calu-3) infected with wild-type Influenza A/Vietnam/1203/2004 (VN1203) virus and mutant viruses PB2-K627E and NS1-trunc124 for mRNA, miRNA, proteomics, lipidomics, and metabolomics data analysis. 

Secondary host-associated viral dataset downloads contain one or more statistically processed (normalization data transformation) quantitative dataset collections resulting in qualitative expression analyses of primary host-pathogen experimental study designs. Leveraging unique high-resolution Omics capabilities for proteomics, metabolomics, lipidomics, and transcriptomics dataset downloads each have a direct relationship to a primary sample submission corresponding to a specific Influenza A virus infection.

Accessible Digital Data Downloads

Transcriptomics

• Expression profiling by array (mRNA) 
• Non-coding RNA profiling by array (miRNA)

Proteomics, Metabolomics, Lipidomics

• Protein quantification by liquid chromatography mass spectrometry (LC-MS)
• Metabolite quantification by gas chromatography mass spectrometry (GC-MS)
• Lipid quantification by liquid chromatography mass spectrometry (LC-MS)

Data Download Reference Citations

1. Anderson, Lindsey N, Eisfeld, Amie J, Waters, Katrina M, and Modeling Host Responses to Understand Severe Human Virus Infections Program Project. Omics-Lethal Human Viruses, Influenza A Experiment ICL103. United States. 2021. PNNL DataHub (Web). DOI: 10.25584/LHVICL103/1661913
2. Eisfeld, A.J., Anderson, L.N., Fan, S. et al. A compendium of multi-omics data illuminating host responses to lethal human virus infections. Sci Data 11, 328 (2024). https://doi.org/10.1038/s41597-024-03124-3

Linked Primary Data Accessions

NCBI BioProject: PRJNA308121

GEO Series: GSE76599 (mRNA transcriptome response), GSE76600 (miRNA transcriptome response)*

MassIVE Accession(s): MSV000079459 (proteome response), MSV000079460 (metabolome response), MSV000080023 (lipidome response)

*Raw measurement data has primary data publication pubmed:34051754

Acknowledgment of Federal Funding

The data described here was funded in whole or in part by the National Institute of Allergy and Infectious Diseases, of the National Institutes of Health under award number U19AI106772 and is a contribution of the "Modeling Host Responses to Understand Severe Human Virus Infections" Project at Pacific Northwest National Laboratory. Data generated by the Omics-LHV Core for proteomics, metabolomics, and lipidomics analyses for were performed at Pacific Northwest National Laboratory in the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the Department of Energy’s (DOE) Office, operating under the Battelle Memorial Institute for the DOE under contract number DE-AC05-76RLO1830. 

Citation Policy

In efforts to enable discovery, reproducibility, and reuse of NIH-funded project dataset citations, we ask that all reuse of project data and metadata download materials acknowledge all primary and secondary dataset citations where applicable and direct corresponding journal articles (Grant U19AI106772) where allowable in accordance with best practices outlined by the FORCE11 Joint Declaration of Data Citation Principles in alignment with NIH acknowledgement requirements.

Data Licensing

CC BY 4.0 (dataset DOI downloads), CC0 1.0 (PNNL DataHub policy default)