Influenza A Experiment IM102
The purpose of this Influenza experiment was to obtain samples for mRNA, miRNA, proteomics, lipidomics, and metabolomics analysis in mouse lung infected with WT A/Anhui/1/2013 (H7N9; 'AH1'), AH - NS1-103F/106M, and AH1 - 691 (ferret adapted virus).
Overall Design: Groups of 22-week-old C57BL/6 mice were infected with the H7N9 Influenza WT A/Anhui/1/2013 (H7N9; 'AH1'), AH - NS1-103F/106M, and AH1 - 691 (ferret adapted virus). Infections were done at 10^4 PFU or time-matched mock infected. There were 5 animals/dose/time point. Lung samples were collected for virus load transcriptional and omics analysis. Transcriptome sample time points: 1, 2, 4 and 7 days post-infection. Mass spectrometry time points: 1, 2, 4 & 7 days post-infection (5 biological replicates).
Processed Omics Data (available at download button):
Dataset downloads contain one or more statistically processed data file related to a lipidomic, transcriptomic, metabolomic, and/or proteomic dataset collection associated with the Influenza experimental study.
Omics Mass Spectrometry
Related Experimental Data
PRIDE Accession: PXD002647 (metabolome response)
Acknowledgment of Federal Funding
The data described here was funded in whole or in part by the National Institute of Allergy and Infectious Diseases, of the National Institutes of Health under award number U19AI106772, and is a contribution of the "Modeling Host Responses to Understand Severe Human Virus Infections" Project at Pacific Northwest National Laboratory. Data generated by the Omics-LHV Core for proteomics, metabolomics, and lipidomics analyses for were performed at Pacific Northwest National Laboratory in the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the Department of Energy’s (DOE) Office, operating under the Battelle Memorial Institute for the DOE under contract number DE-AC05-76RLO1830.