Last updated on 2023-01-30T00:09:57+00:00 by LN Anderson
Ebola Virus Experiment EHUH002
The purpose of this experiment was to evaluate the human host response to Zaire Ebola wild-type virus and mutant virus infection. Samples were obtained from human hepatoma carcinoma cells (HUH-7) infected with Zaire Ebola ΔVP30-WT background for proteome, metabolome, and lipidome expression analysis.
Secondary host-associated viral dataset downloads contain one or more statistically processed (normalization data transformation) quantitative dataset collections resulting in qualitative expression analyses of primary host-pathogen experimental study designs. Leveraging unique high-resolution Omics capabilities for proteomics, metabolomics, and lipidomics dataset download each have a direct relationship to a primary sample submission corresponding to a specific Ebola virus infection.
Accessible Digital Data Downloads
Proteomics, Metabolomics, Lipidomics
- Protein quantification by liquid chromatography mass spectrometry (LC-MS)
- Metabolite quantification by gas chromatography mass spectrometry (GC-MS)
- Lipid quantification by liquid chromatography mass spectrometry (LC-MS)
Anderson, Lindsey N, Eisfeld, Amie J, Waters, Katrina M, and Modeling Host Responses to Understand Severe Human Virus Infections Program Project. Omics-Lethal Human Viruses, Ebola Experiment EHUH002. United States. 2021. PNNL DataHub (Web). DOI: 10.25584/LHVEHUH002/1661906
Linked Primary Data Accessions
MassIVE Accession(s): MSV000081041 (proteome response), MSV000081042 (metabolome response), MSV000081892 (lipidome response)
Acknowledgment of Federal Funding
The data described here was funded in whole or in part by the National Institute of Allergy and Infectious Diseases, of the National Institutes of Health under award number U19AI106772 and is a contribution of the "Modeling Host Responses to Understand Severe Human Virus Infections" Project at Pacific Northwest National Laboratory. Data generated by the Omics-LHV Core for proteomics, metabolomics, and lipidomics analyses for were performed at Pacific Northwest National Laboratory in the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the Department of Energy’s (DOE) Office, operating under the Battelle Memorial Institute for the DOE under contract number DE-AC05-76RLO1830.
In efforts to enable discovery, reproducibility, and reuse of NIH-funded project dataset citations, we ask that all reuse of project data and metadata download materials acknowledge all primary and secondary dataset citations where applicable and direct corresponding journal articles (Grant U19AI106772) where allowable in accordance with best practices outlined by the FORCE11 Joint Declaration of Data Citation Principles in alignment with NIH acknowledgement requirements.
CC BY-SA 4.0 (secondary dataset download DOIs), CC0 1.0 (project metadata and PNNL DataHub policy default)