Category
Description
Omics-LHV, West Nile
Experiment WGCN002
The purpose of this West Nile experiment was to obtain samples for transcriptome analysis in mouse granule cell neurons infected with wild type West Nile virus (WNV-NY99 clone 382, WNVWT) and mutant virus (WNVE218A).
Overall Design: Granule cell neurons from day 6 C57Bl/6J mouse pups were infected with plasmid-derived wild type West Nile virus NY99 clone 382 (WNV-NY99 clone 382, WNVWT) or plasmid-derived West Nile virus NY99 clone 382 isogenic E218A mutant (WNVE218A) virus. Infection were done in triplicate with a multiplicity of infection (MOI) of 250. Time matched mocks were collected in parallel using cell media concentrated through ultracentrifugation and diluted as virus. Time points used were: 1, 8, 12, and 24 hrs post infection (miRNA: 12 hrs only).
See Experiment WGCN003 for corresponding independent biological replicate study.
Processed Omics Data (available at download button):
Dataset downloads contain one or more statistically processed data file related to a lipidomic, transcriptomic, metabolomic, and/or proteomic dataset collection associated with a West Nile experimental study.
Transcriptomics
mRNA
miRNA
Related Experimental Data
BioProject: PRJNA282548, PRJNA282547
GEO: GSE68380 (mRNA transcriptome response), GSE68381 (miRNA transcriptome response)