Omics-LHV, West Nile
The purpose of this West Nile experiment was to obtain samples for transcriptome analysis in mouse granule cell neurons infected with wild type West Nile virus (WNV-NY99 clone 382, WNVWT) and mutant virus (WNVE218A).
Overall Design: Granule cell neurons from day 6 C57Bl/6J mouse pups were infected with plasmid-derived wild type West Nile virus NY99 clone 382 (WNV-NY99 clone 382, WNVWT) or plasmid-derived West Nile virus NY99 clone 382 isogenic E218A mutant (WNVE218A) virus. Infection were done in triplicate with a multiplicity of infection (MOI) of 250. Time matched mocks were collected in parallel using cell media concentrated through ultracentrifugation and diluted as virus. Time points used were: 1, 8, 12, and 24 hrs post infection (miRNA: 12 hrs only).
See Experiment WGCN002 for corresponding independent biological replicate study.
Processed Omics Data (available at download button):
Dataset downloads contain one or more statistically processed data file related to a lipidomic, transcriptomic, metabolomic, and/or proteomic dataset collection associated with a West Nile experimental study.
Related Experimental Data
BioProject: PRJNA282548, PRJNA282547
GEO: GSE68380 (mRNA transcriptome response), GSE68381 (miRNA transcriptome response)