Omics-LHV, Influenza A Experiment ICL106
The purpose of this experiment was to evaluate the host epigenetic response to Influenza A H5N1 virus and MERS-CoV viral infection. Sample data was obtained for human Calu-3 cells infected with Influenza A (H5N1) and MERS-CoV virus for genome binding/occupancy profiling by high throughput sequencing analysis (MeDIP-Seq).
Overall Design: Examination of differential methylation status in Calu-3 cells in 6-well plates were infected with MERS-CoV at an MOI=5 and Influenza A H5N1 at an MOI=3, harvesting 18 hours post infection. Lysates were pooled (3 samples) were clarified from sonicated nuclei and methylated DNA was enriched by using the MethylMiner Methylated DNA Enrichment Kit (ME10025, Invitrogen).
Methylation profiling by high throughput sequencing (MeDIP-Seq)
Related Experimental Data
GEO Accession: GSE108882 (MeDIP-Seq)
SRA Accession: SRP128563 (Differential Methylation During MERS-CoV and H5N1 Infection)