OmicsLHV-ICL106

Dataset Image

Description

Omics-LHV, Influenza A Experiment ICL106

The purpose of this experiment was to evaluate the host epigenetic response to Influenza A H5N1 virus and MERS-CoV viral infection. Sample data was obtained for human Calu-3 cells infected with Influenza A (H5N1) and MERS-CoV virus for genome binding/occupancy profiling by high throughput sequencing analysis (MeDIP-Seq). 

Overall Design: Examination of differential methylation status in Calu-3 cells in 6-well plates were infected with MERS-CoV at an MOI=5 and Influenza A H5N1 at an MOI=3, harvesting 18 hours post infection. Lysates were pooled (3 samples) were clarified from sonicated nuclei and methylated DNA was enriched by using the MethylMiner Methylated DNA Enrichment Kit (ME10025, Invitrogen).

 

Viral Species:

  1. Influenza A /Vietnam/1203/2004 (H5N1, VN1203)
  2. MERS-CoV Hu/Jordan-N3/2012 (EMC)

 

Genomics (Epigenomics)

Methylation profiling by high throughput sequencing (MeDIP-Seq)

 

Related Experimental Data

BioProject: PRJNA428998

GEO Accession: GSE108882 (MeDIP-Seq)

SRA Accession: SRP128563 (Differential Methylation During MERS-CoV and H5N1 Infection)

English
Projects (2)
Publications (1)
People (2)

Lindsey Anderson’s research has been dedicated to the identification and characterization of novel, targeted and non-targeted, functional metabolic interactions using a high-throughput systems biology and computational biology approach. Her expertise in functional metabolism and multidisciplinary...

Dr. Jason McDermott, senior research scientist, has extensive research experience in molecular and structural virology and data resource design, data integration and prediction of biological networks, bridging experimental and computational biology. Currently, his research interests include data...

Data Sources (1)