Dataset Influence of soil depth, moisture, and plants on multi-omic features and carbon chemistry in a calcareous soil: Sequence Data

Please cite as: Naasko K.I., D.T. Naylor, E.B. Graham, S.P. Couvillion, R.E. Danczak, N. Tolic, and C.D. Nicora, et al. 2023. Influence of soil depth, moisture, and plants on multi-omic features and carbon chemistry in a calcareous soil: Sequence Data. [Data Set] PNNL DataHub. https://doi.org/10.25584/PNNLDH/1985150

This file was created by Bill Nelson

GENERAL INFORMATION

1. Title of Dataset: Depth and moisture effects on soil chemistry and microbiome composition 2020 Naasko

2. Contributors:
    Principal Investigator: Kirsten Hofmockel
    Data contributor (creator, producer, etc): Katherine Naasko

3. Information about funding sources supporting the data: DOE BER GSP Soil Microbiome SFA, FWP 

4. Geographic location of data collection: 46°15'04"N, 119°43'43"W, Prosser, WA, USA

5. Data History (single date, range, approximate date) <suggested format YYYY-MM-DD>: 
    A. Reporting Date: 2023-03-10
    B. Data activity type: 
    D. Activity Reporter Contact Information : nels329

DATA & FILE OVERVIEW

1. Data Activity Type Record:
    A. File List: 
        1. 16S_Data-20230103T174740Z-001.zip - 16S amplicon data from 144 samples
        2. ITS_Data-20230104T193619Z-001.zip - fungal ITS amplicon data from 144 samples
    C. Instrument- or software-specific information needed to interpret the data: 
    <include full name and version of software, and any necessary packages or libraries needed to run scripts>
    D. Standards and calibration information, if appropriate: 
    E. Environmental/experimental conditions: 
        WSU Irrigated Agriculture and Research Extension Center in Prosser, WA
        July 20 and 21, 2020
        Three replicates each of 6 treatments (Unirrigated bare, 25% field capacity (FC) bare, 25% FC Alkar, 100% FC Alkar, 25% FC Jose, 100% FC Jose);
        cores subsectioned into 4 depth horizons (0-5cm, 5-15cm, 15-48cm, 48-100cm(max)); collected forward and reverse reads

    F. Describe any quality-assurance procedures performed on the data: 

DATA-SPECIFIC INFORMATION

1. 16S_Data-20230103T174740Z-001.zip - a zip archive of a folder containing 144 gzipped fastq files, a manifest file, and a metadata yml file.

2. ITS_Data-20230104T193619Z-001.zip - a zip archive of a folder containing 144 gzipped fastq files, a manifest file, and a metadata yml file.