Dataset Chitin Decomposition 16S rRNA gene fastq

Please cite as: Nicholas J Reichart, Sheryl Bell, Vanessa A. Garayburu-Caruso, Natalie Sadler, Sharon Zhao, Kirsten Hofmockel. Chitin Decomposition 16S rRNA gene fastq. [Data Set] PNNL DataHub. 10.25584/2475041.

This data is published under a CC0 license. The authors encourage data reuse and request attribution by referencing the above citations for the data package and associated manuscript.

This dataset contains the demultiplexed fastq files for the Soils SFA Chitin Decomposition project in task 2.2. Samples were generated from soil incubated under different moisture levels, with and without chitin. Further processing was done to extract the DNA directly from soil samples or after fluorescence activated cell sorting based on fluorescent signal of activity based probes. The v4 region of the 16S rRNA gene was amplified and sequencing was done using the Illumina MiSeq 2x250 flow cell platform.