Dataset Soil DNA and RNA Virus Sequence Data Recovered Using Different Preparation Methods

Please cite as: Josué A. Rodríguez-Ramos, Amy E. Zimmerman, Ruonan Wu, Sheryl Bell, Trinidad Alfaro, Kirsten Hofmockel, William C. Nelson. Soil DNA and RNA Virus Sequence Data Recovered Using Different Preparation Methods. https://doi.org/10.25584/2583337

This data is published under a CC0 license. The authors encourage data reuse and request attribution by referencing the above citations for the data package and associated manuscript.

To enhance detection of communities of DNA and RNA viruses, we applied different preparation methods to soils collected across a moisture gradient from a grassland field experiment. Analyses included metagenomics and metatranscriptomics of size-fractionated extracellular viruses and total soil, total soil metatranscriptomics with polyadenylation enrichment, and metagenomics of bacteria/archaea as well as eukaryote-enriched samples. DNA virome isolation outperformed total soil metagenomes. Contrastingly, RNA virome isolation and total soil metatranscriptomes performed similarly for viral recovery, though RNA viromes yielded higher-quality genomes. Different preparation methods differed in identifying distinct viral communities and predicted host ranges. Relationships between vOTU diversity and expression by moisture varied for both DNA and RNA vOTUs and method.