Publication - Journal Article High-throughput genetic engineering of nonmodel and undomesticated bacteria via iterative site-specific genome integration

Elmore JR, Dexter GN, Baldino H, Huenemann JD, Francis R, Peabody GL 5th, Martinez-Baird J, Riley LA, Simmons T, Coleman-Derr D, Guss AM, Egbert RG. High-throughput genetic engineering of nonmodel and undomesticated bacteria via iterative site-specific genome integration. Sci Adv. 2023 Mar 10;9(10):eade1285. doi: 10.1126/sciadv.ade1285. Epub 2023 Mar 10. PMID:36897939; PMCID:PMC10005180.

Funding Acknowledgments

This work was supported in part by the U.S. Department of Energy (DOE), Office of Biological and Environmental Research (BER), as part of BER’s Genomic Science Program (GSP), and is a contribution of the Pacific Northwest National Laboratory (PNNL) Secure Biosystems Design Science Focus Area “Persistence Control of Engineered Functions in Complex Soil Microbiomes.” Additional support was provided by the DARPA Synergistic Discovery and Design program (contract # HR0011045664). PNNL is a multiprogram national laboratory operated by Battelle for the DOE under contract DE-AC05-76RL01830. This work was authored in part by the Oak Ridge National Laboratory, which is managed by UT-Battelle LLC, for the U.S. DOE under contract DE-AC05-00OR22725. Funding was provided in part by the U.S. DOE, Office of Energy Efficiency and Renewable Energy Bioenergy Technologies Office (BETO) to the Agile BioFoundry. This work was in part supported by the Center for Bioenergy Innovation, U.S. DOE Bioenergy Research Center, supported by the Office of Biological and Environmental Research in the DOE Office of Science.

Last updated on 2023-03-14T23:13:24+00:00 by LN Anderson