Data Source - Instrument Nikon Eclipse TE2000-E Epifluorescence Microscope

The Nikon Eclipse Ti2-E is a motorized and intelligent model for advanced imaging applications. Compatible with PFS, auto correction collar, and external phase contrast system. The base of choice for live-cell imaging, high-content applications, confocal and super-resolution.As research trends evolve towards large-scale, systems-level approaches, there is an increasing demand for faster data acquisition and higher throughput capabilities. The Ti2, with its unprecedented 25mm field of view, provides the next level of scalability, enabling researchers to truly maximize the utility of large-format detectors and future-proof their core imaging platform as camera technologies continue to develop at a rapid pace. Motorized and intelligent model for advanced imaging applications. Compatible with PFS, auto correction collar, and external phase contrast system. The base of choice for live-cell imaging, high-content applications, confocal and super-resolution. A compact epi-fluorescence illuminator designed for large FOV imaging is equipped with a quartz fly-eye lens and provides high transmittance across a broad spectrum, including UV. Large diameter fluorescence filters with hard coatings deliver large FOV images with a high signal-to-noise ratio. The Lambda series objectives, utilizing Nikon's proprietary Nano Crystal Coat technology, are perfect for demanding, low-signal, multi-channel fluorescence imaging that requires high transmission and aberration correction over a wide wavelength range. Combined with new fluorescence filter cubes that offer improved fluorescence detection and stray light countermeasures such as the Noise Terminator, the Lambda series objectives demonstrate their power in weak signal observations such as single-molecule imaging and even luminescence-based applications.

Data Source Contributions

• LN Anderson (Domain Biocuration): Data curation, Writing – original draft, Writing – review & editing

Instrument Data Source Profile

Instrument Name:     Nikon Eclipse TE2000-E Epifluorescence Microscope

Model:                         Eclipse Ti2-E

Platform Type:           Epifluorescence Microscope

Data Type:                   Brightfield and Fluorescent Imaging

Software:                    ImageJ

Vendor Brochure:      Nikon Ti2-E Inverted Raman Microscope

Instrument Group:     Microscopy, Fluorescence Microscopy, Epifluorescence Microscopy

Topic Areas:               Imaging, Optical Micrograph, Optical Microscopes, Biogeochemical Transformations, Flow & Transport

Explore this capability and more at EMSL's Chemical Imaging resources page under Pore Scale Micromodels.

Acknowledgements

This work was supported in part by the Earth and Biological Sciences Directorate (EBSD) at Pacific Northwest National Laboratory (PNNL), a multiprogram national laboratory managed by the Battelle Memorial Institute, operating under the U.S. Department of Energy (DOE) contract DE-AC05-76RL01830. User capabilities described here reflect collaborations with the Environmental Molecular Sciences Laboratory (EMSL), a DOE Office of Science (SC-3) user facility operating under the Contract No. DE-AC05-76RL01830.

Terms of Use

Scientists who publish results of research using EMSL resources, capabilities, and resulting data are required to include an acknowledgment of EMSL Policies in any publications. Learn more about user facility data management resources at the Office of Science Program page.