This data set provides ITS fungal community composition via DNA sequence analysis at the time of peat coring at the South End bog in 2013. These samples were collected outside the experimental enclosures and are pre-treatment with no experimental manipulation. These are part of the Spruce and Peatlands Under Changing Environments (SPRUCE) experiment. Samples were extracted using a Qiagen Power Soil Kit according to manufacture protocols. Sequencing was completed at Argonne National Lab Environmental Sample Preparation and Sequencing Facility (https://www.anl.gov/bio/environmental-sample-preparation-and-sequencing-facility), following the protocol for Earth Microbiome described by Caporaso, J. et al 2012.