Category
Description
Human Host Cellular Response to HCoV-229E Infection Transcriptomics (ACS-DP1)
The purpose of this experiment was to evaluate the human host cellular response to wild-type Human coronavirus strain 229E (HCoV-229E) infection. Sample data was obtained for mock and infected immortalized human lung epithelial cells (A549) (MOI 5), immortalized human lung fibroblasts cells (MRC5) (MOI5), and primary human airway epithelial (HAE) (MOI 3) cells from lung tissue. Sample data was acquired using an Illumina HiSeq 2000 sequencer system and processed for RNA sequencing (RNA-Seq) expression analysis.
Accessible Digital Data Downloads
The repository contains the following folders and files:
- Experimental_Metadata: Folder with additional information to understand the experimental data
- Raw Counts: Folder with files containing the sequence counts across all samples
- Processed Data: Folder with files containing normalized sequence counts and differential expression statistics
- Legal: Additional files with legal information
Total Download Size: 3.8 MB, zipped
Linked Publications
Sarkar S, S Feng, HD Mitchell, MR Berger, T Zhang, IK Attah, CM Hutchinson-Bunch, VN Prozapas, K Engbrecht, S King, AC Sims and JT Melchior. 2025. "Human coronavirus-229E hijacks key host-cell RNA processing complexes for replication." Journal of Proteome Research, 24(10), 5026-5045. https://doi.org/10.1021/acs.jproteome.5c00400
Linked Primary Data
Primary RNA-Seq raw measurement data are openly accessible for download at the Gene Expression Omnibus (GEO) community repository under the accessions GSE279271 (H549 & MRC5) and GSE279463 (HAE).
Funding Acknowledgments
The research data described here was funded in whole or in part by the Predictive Phenomics Initiative (PPI) at Pacific Northwest National Laboratory (PNNL). This work was conducted under the Laboratory Directed Research and Development Program at PNNL. PNNL is a multiprogram national laboratory operated by Battelle for the DOE under Contract No. DE-AC05-76RL01830.
Citation Policy
In efforts to enable discovery, reproducibility, and reuse of PPI-funded project dataset citations in accordance with best practices (as outlined by the FORCE11 Data Citation Principles), we ask that all reuse of project data and metadata download materials acknowledge all primary and secondary dataset citations and corresponding journal articles where applicable.
Data Licensing