Dataset - Proteomics S. elongatus PCC 7942 Carbon Metabolism Proteomics (MC-DP2)

Dataset Description

The purpose of this experiment was to examine the redox proteome of S. elongatus PCC 7942 CscB/SPS under different light conditions (light and dark) and culture conditions (dense and dilute optical density). Samples were processed using a resin-assisted capture (RAC) workflow with TMT labeling to enrich and quantify protein cysteines which were differentially oxidized under four conditions. The datasets were generated by a Q Exactive Plus Orbitrap Mass Spectrometer coupled with a Waters nanoAcquity UPLC, then searched by MSGF+ for downstream redox PTM analysis.

Data Download Reference Citation

Cheung, Margaret; Xiaolu Li, Tong Zhang, Pavlo Bohutskyi and Katrina Waters. (2025) S. elongatus PCC 7942 Carbon Metabolism Proteomics (MC-DP2). https://doi.org/10.25584/PPI/2475747

Accessible Digital Data Downloads

The repository contains the following folders and files:

• MC_DP2_SampleMetadata.xlsx: Contains sample metadata information including descriptors, experimental conditions, cell lines (if applicable)
• MC_DP2_Syn Protein Data.xlsx: Normalized abundance and statistics for a monoculture of S. elongatus using both global and redox proteomics

Total Download Size: 3.6 MB, zipped

Linked Publications

1. Johnson, C. G. M., Johnson, Z., Mackey, L. S., Li, X., Sadler, N. C., Zhang, T., Qian, W.-J., Bohutskyi, P., Feng, S., & Cheung, M. S. (2025). Multi-Omics Reveals Temporal Scales of Carbon Metabolism in Synechococcus Elongatus PCC 7942 Under Light Disturbance. PRX Life, 3(3). DOI: 10.1103/l2dp-kw2t

Linked Primary Data

Primary liquid chromatography-mass spectrometry (LC-MS) raw measurement data are openly accessible for download at the Mass Spectrometry Interactive Virtual Environment (MassIVE) community repository under the accession MSV000096184.

Funding Acknowledgments

The research data described here was funded in whole or in part by the Predictive Phenomics Initiative (PPI) at Pacific Northwest National Laboratory (PNNL). This work was conducted under the Laboratory Directed Research and Development Program at PNNL. A portion of this research was performed in the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the U.S. Department of Energy (DOE) Office of Science located at PNNL. PNNL is a multiprogram national laboratory operated by Battelle for the DOE under Contract No. DE-AC05-76RL01830.

Citation Policy

In efforts to enable discovery, reproducibility, and reuse of PPI-funded project dataset citations in accordance with best practices (as outlined by the FORCE11 Data Citation Principles), we ask that all reuse of project data and metadata download materials acknowledge all primary and secondary dataset citations and corresponding journal articles where applicable.

Data Licensing

Creative Commons Attribution 4.0 International (CC BY 4.0)