Dataset - Proteomics Human Host Cellular Response to HCoV-229E Infection Proteomics, Whole Cell Lysate (ACS-JM-DP5)

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Chemical & Biological Signatures Science
Human Health
Predictive Phenomics
Mass Spectrometry
Proteome Integral Solubility Alteration
Limited Proteolysis
Global Analysis
Tandem Mass Tag
TMT18
Homo sapiens
HCoV-299E
Time Sampled Measurement Datasets

Dataset Description

The purpose of this experiment was to evaluate the human host cellular response to wild type human coronavirus strain 229E (HCoV-229E) infection. Sample data was obtained for mock-infected and immortalized human lung fibroblasts (MRC-5) (MOI 5). Whole cell lysates were collected at 8, 16, and 24 hours post infection and were processed for proteomic analysis (abundance-based proteomics, limited proteolysis (LiP) structural proteomic analysis, and proteome integral solubility alteration (PISA) structural proteomic analysis. LFQ and TMT18 "whole cell" data packaging activities for LiP assays involving mock and infected experiments using MRC5 cells.

Data Download Reference Citation:

Sims, Amy C; Zhang, Tong; Melchior, J; & Waters, Katrina M (2025). Human Host Cellular Response to HCoV-229E Infection Proteomics, Whole Cell Lysate (ACS-JM-DP5)

Accessible Digital Data Downloads

The repository contains the following folders and files:

  • ACS-JM-DP5_SampleMetadata.xlsx: Contains sample metadata information including descriptors, experimental conditions, cell lines (if applicable)
  • ACS-JM-DP5_PISA.xlsx: Contains normalized abundance PISA data for the MRC5 cell line
  • ACS-JM-DP5_LiP.xlsx: Contains normalized abundance LiP data and statistics for the MRC5 cell line

Total Download Size: 25.3 MB, zipped

Linked Primary Data

Primary mass spectrometry data can be found in MassIVE. The PISA data is located here: MSV000099899. LiP is available here: MSV000099901.

The research data described here was funded in whole or in part by the Predictive Phenomics Initiative (PPI) at Pacific Northwest National Laboratory (PNNL). This work was conducted under the Laboratory Directed Research and Development Program at PNNL. PNNL is a multiprogram national laboratory operated by Battelle for the DOE under Contract No. DE-AC05-76RL01830.

Citation Policy

In efforts to enable discovery, reproducibility, and reuse of PPI-funded project dataset citations in accordance with best practices (as outlined by the FORCE11 Data Citation Principles), we ask that all reuse of project data and metadata download materials acknowledge all primary and secondary dataset citations and corresponding journal articles where applicable.

Data Licensing

Creative Commons Attribution 4.0 International (CC BY 4.0)

English
Projects (3)
Predictive Phenomics Initiative Project Dataset Catalog Collection
Structural Profiling of the Molecular Interactome to Define Phenotype
Manipulation of Host Signature Expression Following Respiratory Virus Infection
People (3)
John Melchior

John is an accomplished lipid biochemist and structural biologist with an interest in understanding molecular pathology of disease. He earned his Ph.D. from Wake Forest School of Medicine where he received training in lipid biochemistry under the late Dr. Lawrence Rudel studying the role of low...

Amy Sims

Amy Sims, PhD is a Biomedical Scientist in the Chemical and Biological Signatures Division of the National Security Directorate at the Pacific Northwest National Laboratory (PNNL) in Richland, WA. She earned her Ph.D. from Vanderbilt University Medical Center and worked with Ralph Baric, PhD at the...

Tong Zhang

Dr. Tong Zhang is a scientist in the Biological Sciences Division at the Pacific Northwest National Laboratory (PNNL). His research focuses on using mass spectrometry-based proteomics to understand fundamental biology and improve human health. His current projects include developing redox proteomics...