Dataset - Proteomics Human Lung Cellular Response to HCoV-229E Infection, Post-Translational Modification Proteomics Time Course (ACS-TZ-DP6)

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Dataset Description

The purpose of this experiment was to evaluate the human host cellular response to wild type human coronavirus strain 229E (HCoV-229E) infection. Sample data was obtained for mock-infected and HCoV-229E infected immortalized human lung epithelial cells (A549) (MOI 3) and immortalized human lung fibroblasts (MRC-5) (MOI 3). Whole cell lysates were collected at 8, 16, and 24 hours post infection and were processed for post translational modification (PTM) proteomics analysis to determine the HCoV-229E phosphoproteome, redox proteome, and acetylation proteome. 

Data Download Reference Citation:

Sims, Amy, T Zhang & KM Waters (2026). Human Lung Cellular Response to HCoV-229E Infection Post-Translational Modification Proteomics Time Course (ACS-TZ-DP6)

Accessible Digital Data Downloads

The repository contains the following folders and files:

  • ACS-TZ-DP6_SampleMetadata.xlsx: Contains sample metadata information including descriptors, experimental conditions, cell lines (if applicable)
  • ACS-TZ-DP6_PTMs_ExpressionData.xlsx: Contains normalized abundance PTM data and statistics for both the A549 and MRC5 cell lines using acetyl, global, redox and phospho PTMs. 

Total Download Size: 115 MB, zipped

Linked Primary Data

Primary mass spectrometry data can be found in MassIVE: MSV000100308 (A549 and acetyl PTMs), MSV000100311 (A549 and global PTMs), MSV000100313 (A549 and redox PTMs), MSV000100324 (A549 and phospho PTMs), MSV000100295 (MRC5 and acetyl PTMs), MSV000100296 (MRC5 and global PTMs), MSV000100299 (MRC5 and redox PTMs), MSV000100300 (MRC5 and phospho PTMs). 

Funding Acknowledgments

The research data described here was funded in whole or in part by the Predictive Phenomics Initiative (PPI) at Pacific Northwest National Laboratory (PNNL). This work was conducted under the Laboratory Directed Research and Development Program at PNNL. PNNL is a multiprogram national laboratory operated by Battelle for the DOE under Contract No. DE-AC05-76RL01830.

Citation Policy

In efforts to enable discovery, reproducibility, and reuse of PPI-funded project dataset citations in accordance with best practices (as outlined by the FORCE11 Data Citation Principles), we ask that all reuse of project data and metadata download materials acknowledge all primary and secondary dataset citations and corresponding journal articles where applicable.

Data Licensing

Creative Commons Attribution 4.0 International (CC BY 4.0)

Language English

English
Projects (3)
Predictive Phenomics Initiative Project Dataset Catalog Collection
Structural Profiling of the Molecular Interactome to Define Phenotype
Manipulation of Host Signature Expression Following Respiratory Virus Infection
People (1)
Tong Zhang

Dr. Tong Zhang is a scientist in the Biological Sciences Division at the Pacific Northwest National Laboratory (PNNL). His research focuses on using mass spectrometry-based proteomics to understand fundamental biology and improve human health. His current projects include developing redox proteomics...