Dataset Human Lung Fibroblast Response to HCoV-229E Infection, Top-down Proteomics of Histones (ACS-TZ-DP7)

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Dataset Description

The purpose of this experiment was to evaluate the human host cellular response to wild type human coronavirus strain 229E (HCoV-229E) infection, specifically how histones are modified following infection. Sample data was obtained from mock-infected and HCoV-229E-infected immortalized human lung fibroblasts (MRC-5) (MOI 3). Whole cell lysates were collected at 24 hours post infection and histones from all samples were enriched and were processed for proteoform identification analysis.

Data Download Reference Citation:

Zhang, Tong; & Waters, Katrina M (2025). Human Lung Fibroblast Response to HCoV-229E Infection, Top-down Proteomics of Histones (ACS-TZ-DP7)

Accessible Digital Data Downloads

The repository contains the following folders and files:

  • ACS-TZ-DP7_SampleMetadata.xlsx: Contains sample metadata information including descriptors, experimental conditions, and viral titers
  • ACS-TZ-DP7_histone protein data.xlsx: Contains normalized, statistics and feature data for the histone proteoform analysis of the MRC5 cell line

Total Download Size: 216 KB, zipped

Linked Publication

Ives AN, SM Thibert, MR Berger, MJ Gaffrey, HD Mitchell, SM Williams, M Zhou, K Waters, AC Sims, and T Zhang. 2026. “Human coronavirus 229E infection alters histone proteoforms.” Journal of Proteome Research. https://doi.org/10.1021/acs.jproteome.5c01015

Linked Primary Data

Primary mass spectrometry data can be found in MassIVE: MSV000099293

The research data described here was funded in whole or in part by the Predictive Phenomics Initiative (PPI) at Pacific Northwest National Laboratory (PNNL). This work was conducted under the Laboratory Directed Research and Development Program at PNNL. PNNL is a multiprogram national laboratory operated by Battelle for the DOE under Contract No. DE-AC05-76RL01830.

Citation Policy

In efforts to enable discovery, reproducibility, and reuse of PPI-funded project dataset citations in accordance with best practices (as outlined by the FORCE11 Data Citation Principles), we ask that all reuse of project data and metadata download materials acknowledge all primary and secondary dataset citations and corresponding journal articles where applicable.

Data Licensing

Creative Commons Attribution 4.0 International (CC BY 4.0)

English
Projects (3)
Predictive Phenomics Initiative Project Dataset Catalog Collection
Structural Profiling of the Molecular Interactome to Define Phenotype
Manipulation of Host Signature Expression Following Respiratory Virus Infection
People (3)
Tong Zhang

Dr. Tong Zhang is a scientist in the Biological Sciences Division at the Pacific Northwest National Laboratory (PNNL). His research focuses on using mass spectrometry-based proteomics to understand fundamental biology and improve human health. His current projects include developing redox proteomics...

John Melchior

John is an accomplished lipid biochemist and structural biologist with an interest in understanding molecular pathology of disease. He earned his Ph.D. from Wake Forest School of Medicine where he received training in lipid biochemistry under the late Dr. Lawrence Rudel studying the role of low...

Amy Sims

Amy Sims, PhD is a Biomedical Scientist in the Chemical and Biological Signatures Division of the National Security Directorate at the Pacific Northwest National Laboratory (PNNL) in Richland, WA. She earned her Ph.D. from Vanderbilt University Medical Center and worked with Ralph Baric, PhD at the...