Publication - Journal Article Sorgoleone degradation by sorghum-associated bacteria; an opportunity for enforcing plant growth promotion (pre-print)

https://doi.org/10.1101/2023.05.26.542311
Description

Metabolite exchange between plant roots and their associated rhizosphere microbiomes underpins plant growth promotion by microbes. Sorghum bicolor is a cereal crop that feeds animals and humans and is used for bioethanol production. Its root tips exude large amounts of a lipophilic benzoquinone called sorgoleone. Sorgoleone is an allelochemical that suppresses the growth of competing plant seedlings and is mineralized by microbes in soil. As an avenue to understand how sorghum and its root microbiome may be connected through root exudates, we identified the molecular determinants of microbial sorgoleone degradation and the distribution of this trait among microbes. We isolated and studied from sorghum-associated soils, three bacterial strains classified as Acinetobacter, Burkholderia, and Pseudomonas species that grow with sorgoleone as a sole carbon and energy source. The discovery of bacteria that grow on a compound like sorgoleone that is plant specific and not widely distributed in the environment, provides an opportunity to study how a plant exudate can enforce the development of a rhizosphere specific microbiome for the mutual benefit of plant and microbe.

 

Reference Citation

Sorgoleone degradation by sorghum-associated bacteria; an opportunity for enforcing plant growth promotion. Yasuhiro Oda, Joshua R. Elmore, William C. Nelson, Andrew Wilson, Yuliya Farris, Ritu Shrestha, Citlali Fonseca Garcia, Dean Pettinga, Aaron J. Ogden, Henri Baldino, William G. Alexander, Adam M Deutschbauer, Catalina Vega Hurtado, Jason E. McDermott, Adam M. Guss, Devin Coleman-Derr, Ryan McClure, Caroline S. Harwood, Robert G. Egbert. bioRxiv. 2023.05.26.542311; doi: https://doi.org/10.1101/2023.05.26.542311

PerCon SFA
High Throughput Sequencing
Sorghum bicolor
Whole Genome Sequencing
RNA Sequence Analysis
Projects (1)
Project
Persistence Control of Engineered Functions in Complex Soil Microbiomes (PerCon SFA), Secure Biosystems Design Project

Last updated on 2023-02-23T19:37:46+00:00 by LN Anderson PerCon SFA Project Publication Experimental Data Catalog The Persistence Control of Engineered Functions in Complex Soil Microbiomes Project (PerCon SFA) at Pacific Northwest National Laboratory ( PNNL ) is a Genomic Sciences Program...

Scientific Discovery
Biology
Microbiome Science
Earth System Science

  1. Datasets

    3
English
Datasets (1)
Whole Genome Sequencing of Sorgoleone Promoting Rhizobacteria Isolates
People (7)
Joshua Elmore

Dr. Joshua Elmore is a Chemical Engineer at Pacific Northwest National Laboratory. He received his Ph. D. from the University of Georgia in the Department of Molecular Biology and Biochemistry studying the mechanisms of CRISPR/Cas microbial immune systems in hyperthermophilic Archaea. He was a...

William C Nelson

Dr. Nelson is a computational scientist at PNNL. Since coming to PNNL, he has focused on methods to determine precise understanding of microbial community structure and function through the resolution of individual genome sequences from metagenomic data, and exploration of variation between...

Yuliya Farris

PNNL Publications 2020 Ogden A.J., T.W. Wietsma, T.E. Winkler, Y. Farris, G.L. Myers, and A. Ahkami. 2020. "Dynamics of Global Gene Expression and Regulatory Elements in Growing Brachypodium Root System." Scientific Reports 10. PNNL-SA-145907. doi:10.1038/s41598-020-63224-z 2019 Boiteau R.M., S.J...

Jason McDermott

Dr. Jason McDermott, senior research scientist, has extensive research experience in molecular and structural virology and data resource design, data integration and prediction of biological networks, bridging experimental and computational biology. Currently, his research interests include data...

Ryan McClure

Publications: 2018 Khan NE, Y Maezato, RS McClure, CJ Brislawn, JM Mobberley, NG Isern, WB Chrisler, LM Markillie, BM Barney, HS Song, WC Nelson, and HC Bernstein. 2018. "Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture." Scientific...

Robert Egbert

Biography I am a biological engineer working to advance high-throughput biological engineering for rapid optimization of complex gene clusters and dynamical systems in microbes through pooled genomic library assembly, high-throughput sequencing and variant scoring. I utilize machine learning...

Caroline (Carrie) Harwood

Short Biography Caroline (Carrie) Harwood received her Ph.D. in microbiology from the University of Massachusetts and completed postdoctoral work at Yale University. She held academic appointments at Cornell University and the University of Iowa before moving to the University of Washington in 2005...

Institutions (4)
Lawrence Berkeley National Laboratory
University of California, Berkeley
Oak Ridge National Laboratory
University of Washington