Plot of molar ellipticity at 222 nm versus concentration of free La3+ for LanM D5E bound to lanthanum, used to calculate the LanM D5E-La3+ binding affinity (Kd).

Raw chromatographs for the HisTrap and SEC columns run to purify LanM D3E.

Raw UV/Vis data measuring XO fluorescence as a function of increased [La3+]

Plot of molar ellipticity at 222 nm versus concentration of free Y3+ for LanM D5E bound to yttrium, used to calculate the LanM D5E-Y3+ binding affinity (Kd).

Raw NMR HSQC spectra for LanM variants bound to La3+

Raw chromatographs for the HisTrap and SEC columns run to purify LanM D5E.

Raw UV/Vis data measuring XO fluorescence as a function of increased [La3+]

Raw chromatographs for the HisTrap and SEC columns run to purify LanM WT.

Plot of molar ellipticity at 222 nm versus concentration of free La3+ for LanM WT bound to lanthanum, used to calculate the LanM WT-La3+ binding affinity (Kd).

Raw NMR HSQC spectra for LanM variants bound to Y3+

Plot of molar ellipticity at 222 nm versus concentration of free La3+ for LanM D1ED5E bound to lanthanum, used to calculate the LanM D1ED5E-La3+ binding affinity (Kd).

Plot of molar ellipticity at 222 nm versus concentration of free Y3+ for LanM D3ED9E bound to yttrium, used to calculate the LanM D3ED9E-Y3+ binding affinity (Kd).

Plot of molar ellipticity at 222 nm versus concentration of free La3+ for LanM D3ED9E bound to lanthanum, used to calculate the LanM D3ED9E-La3+ binding affinity (Kd).

Plot of molar ellipticity at 222 nm versus concentration of free Y3+ for LanM D1ED5E bound to yttrium, used to calculate the LanM D1ED5E-Y3+ binding affinity (Kd).

Plot of molar ellipticity at 222 nm versus concentration of free Y3+ for LanM WT bound to yttrium, used to calculate the LanM WT-Y3+ binding affinity (Kd).