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Soil microorganisms play fundamental roles in cycling of soil carbon, nitrogen, and other nutrients, yet we have a poor understanding of how soil microbiomes are shaped by their nutritional and physical environment. In this study, we investigated the successional dynamics of a soil microbiome during...
The novel fungal strain, Fusarium sp. DS 682, was isolated from the rhizosphere of the perennial grass, Bouteloua gracilis , at the Konza Prairie Biological Station in Kansas. This fungal strain is common across North American grasslands and is resilient to environmental fluctuations. The draft...
As part of the Pacific Northwest National Laboratory’s (PNNL) Science Focus Area program, we are investigating the impact of environmental change on microbial community function in grassland soils. Three grassland soils, representing different moisture regimes, were selected for ultra-deep...
The soil microbiome is central to the cycling of carbon and other nutrients and to the promotion of plant growth. Despite its importance, analysis of the soil microbiome is difficult due to its sheer complexity, with thousands of interacting species. Here, we reduced this complexity by developing...

The rhizosphere represents a dynamic and complex interface between plant hosts and the microbial community found in the surrounding soil. While it is recognized that manipulating the rhizosphere has the potential to improve plant fitness and health, engineering the rhizosphere microbiome through...

Agriculture is the largest source of greenhouse gases (GHG) production. Conversion of nitrogen fertilizers into more reduced forms by microbes through a process known as biological nitrification drives GHG production, enhances proliferation of toxic algal blooms, and increases cost of crop...

A major challenge in biotechnology and biomanufacturing is the identification of a set of biomarkers for perturbations and metabolites of interest. Here, we develop a data-driven, transcriptome-wide approach to rank perturbation-inducible genes from time-series RNA sequencing data for the discovery...

Last updated on 2023-02-23T19:37:46+00:00 by LN Anderson PerCon SFA Project Publication Experimental Data Catalog The Persistence Control of Engineered Functions in Complex Soil Microbiomes Project (PerCon SFA) at Pacific Northwest National Laboratory ( PNNL ) is a Genomic Sciences Program...

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Dr. Joshua Elmore is a Chemical Engineer at Pacific Northwest National Laboratory. He received his Ph. D. from the University of Georgia in the Department of Molecular Biology and Biochemistry studying the mechanisms of CRISPR/Cas microbial immune systems in hyperthermophilic Archaea. He was a...

Elise Van Fossen is a Post-Doctorate Research Associate in the Biological Sciences Division. Her research focuses on developing synthetic and chemical biology techniques for microorganism engineering.

The innovative Elstar™ electron column forms the basis of the Helios NanoLab’s outstanding high resolution imaging performance. The Elstar features unique technologies, such as constant power lenses for higher thermal stability, electrostatic scanning for faster, higher accurate imaging, and unique...

Stanford Synchrotron Radiation Lightsource Experimental Station 14-3b is a bending magnet side station dedicated to X-Ray Imaging and Micro X-Ray Absorption Spectroscopy of biological, biomedical, materials, and geological samples. Station 14-3b is equipped with specialized instrumentation for XRF...

The Nikon Eclipse Ti2-E is a motorized and intelligent model for advanced imaging applications. Compatible with PFS, auto correction collar, and external phase contrast system. The base of choice for live-cell imaging, high-content applications, confocal and super-resolution.As research trends...

Please cite as : Anderson L.N., W.C. Nelson, J.E. McDermott, R. Wu, S.J. Fansler, Y. Farris, and J.K. Jansson, et al. 2020. IA-TmG.1.0 (Metagenome, IA). [Data Set] PNNL DataHub. https://doi.org/10.25584/IATmG1/1635005 To enable a comprehensive survey of the metabolic potential of complex soil...
Please cite as : Anderson L.N., W.C. Nelson, J.E. McDermott, R. Wu, S.J. Fansler, Y. Farris, and J.K. Jansson, et al. 2020. KS-TmG.1.0 (Metagenome, KS). [Data Set] PNNL DataHub. https://doi.org/10.25584/KSTmG1/1635004 To enable a comprehensive survey of the metabolic potential of complex soil...