The data package consists of isolated pancreatic islets from adult male C57BL6/J mice treated with IL-1β, IFNγ or IL-1β + IFNγ for 6 h and submitted for scRNA-seq ( Table S1 ). This study focused on understanding the heterogeneity of the cytokine-mediated response. Data contributors: Jennifer S...

This data package consists of data from parallel experiments compared to Pck001 but performed with EndoC-βH1 cells instead of human islets. Cells were treated with IFNγ + IL-1β for 48 h and submitted to RNA-seq (n=5), ATAC-seq (n=5), DNA methylation (n=5), and H3K27Ac ChIP-seq (n=4). In addition...

This data package consists of data from paired human islets treated with IFNγ + IL-1β for 48 h and submitted to RNA-seq (n=5), ATAC-seq (n=5), UMI-4C18 (n=2 or 3, depending on the viewpoint), and ChIP-seq for histone H3 lysine 27 acetylation (H3K27Ac) (n=4) ( Table S1 ). Islets across the study...

This data package consists of data from MIN6 cells (n=3) treated with IFNγ + IL-1β + TNFα with or without DFMO or N1,N11-diethylnorspermine (DENSpm) and submitted to RNA-seq ( Table S1 ). DENSpm is an inhibitor of ornithine decarboxylase (ODC) and polyamine oxidase (PAOX), both enzymes in the...

This data package consists of mouse MIN6 cells treated with IL-1β + IFNγ + TNFα for 0 min, 15 min, 2 hr, and 24 hr, alongside an untreated 24 hr control, and submitted to global and PTM proteomics (carbamylation and acetylation) analysis (n=3) ( Table S1 ). This was designed to characterize the...

This data package consists of data from human islets (n=6) treated with IFNγ + IL-1β for 24 h and subjected to top-down proteomics ( Table S1 ). Data contributors: Ashley N. Ives, Wei-Jun Qian & James M. Fulcher: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA...

The data package consists of RNA-seq data from human islet-derived exosomes post IFNγ + IL-1β treatment of human islets (n=5) for 24 h ( Table S1 ). Data contributors: Preethi Krishnan& Carmella Evans-Molina: Department of Diabetes Immunology, Beckman Research Institute, City of Hope, Duarte, CA...

This data package consists of RNA-seq data from human islets (n=10) treated with IFNγ + IL-1β for 24 h ( Table S1 ). Data contributors: Wenting Wu & Carmella Evans-Molina: Center for Diabetes and Metabolic Diseases, Indiana University School of Medicine, Indianapolis, IN, USA. Farooq Syed: Center...

This data package consists of data from human islets (n=8) treated with IFNγ + IL-1β for 24 h and submitted to phosphoproteomics ( Table S1 ). Data contributor: Wei-Jun Qian: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, 99354, USA. Data repository: PXD011857...

It consists of human islets (n=10, subdivided into 2 batches) treated with IFNγ + IL-1β for 24 h and submitted to proteomics, lipidomics and metabolomics in the same samples ( Table S1 ) Data contributors: Ernesto S. Nakayasu, Jennifer E. Kyle, Young-Mo Kim, Soumyadeep Sarkar & Thomas O. Metz...

The data package consists of data from iPSC: HEL115.6-derived islet-like aggregates treated with IFNα for 24 h and submitted to RNA-seq (n=5) and scRNA-seq (n=5). ( Table S1 ). Data contributors: Arturo Roca-Rivada, Xiaoyan Yi & Décio L. Eizirik: ULB Center for Diabetes Research, Medical Faculty...

This data package consists of data from MIN6 cells (n=4) treated with IL-1β + IFNγ + TNFα for 24 h post-RNAi targeting ADP-ribosylhydrolase ARH3 (Adprhl2), and submitted to proteomics analysis ( Table S1 ). ARH3 is a hydrolase that keeps cellular ADP-ribosylation levels in check and is regulated by...

This data package consists of data from β-TC-6 cells treated with IFNγ + IL-1β for 4, 8 or 24 h (n=4) and submitted to enrichment of cysteine-containing peptides (redox), phosphopeptides and acetylated peptides. This enabled the analysis of proteomics, redox cysteine, phosphoproteomics and...

The data package consists of RNA-seq datasets of EndoC-βH1 cells treated in parallel with IFNα, IFNγ, IL-1β and TNFα for 24 h and submitted to RNA-seq (n=4) ( Table S1 ) Data contributors: Arturo Roca-Rivada, Xiaoyan Yi & Décio L. Eizirik: ULB Center for Diabetes Research, Medical Faculty...

This data package consists of data from human islets (n=5) treated with IFNγ + IL-1β with or without difluoromethylornithine (DFMO) and submitted to RNA-seq and proteomics analysis ( Table S1 ). DFMO is an inhibitor of ornithine decarboxylase (ODC), an enzyme of the polyamine pathway. Therefore...