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Dataset
Nanopore Readable Activity Probes for Ribosomal Inactivating Protein (RIP) Toxins

Ribosome inactivating proteins (RIPs) such as ricin and abrin depurinate an adenine base in the sarcin/ricin loop in the large ribosomal subunit, leading to inhibtion of protein synthesis and cell death. Here, we demonstrate that RIP toxin activity can be detected via nanopore-based DNA sequencing...

Dataset
WA-TmG.2.0 (Metagenome, WA)

Please cite as : Anderson L.N., R. Wu, W.C. Nelson, J.E. McDermott, K.S. Hofmockel, and J.K. Jansson. 2021. WA-TmG.2.0 (Metagenome, WA). [Data Set] PNNL DataHub. https://doi.org/10.25584/WATmG2/1770324​​​​​​​ Soil samples were collected in triplicate in the fall of 2017 across the three grassland...

Biology
Earth System Science
Dataset
Iso-VIG14.1.0 (Metagenome Derived Viral Genomes, WA/IA/KS)

Please cite as : Anderson L.N., R. Wu, W.C. Nelson, J.E. McDermott, K.S. Hofmockel, and J.K. Jansson. 2021. Iso-VIG14.1.0 (Metagenome Derived Viral Genomes, WA/IA/KS). [Data Set] PNNL DataHub. https://doi.org/10.25584/IsoVIG14/1770369 Soil samples were collected in triplicate in the Fall of 2017...

Biology
Earth System Science
Dataset
IA-TmG.2.0 (Metagenome, IA)

Please cite as : Anderson L.N., R. Wu, W.C. Nelson, J.E. McDermott, K.S. Hofmockel, and J.K. Jansson. 2021. IA-TmG.2.0 (Metagenome, IA). [Data Set] PNNL DataHub. https://doi.org/10.25584/IATmG2/1770333 Soil samples were collected in triplicate in the fall of 2017 across the three grassland locations...

Biology
Earth System Science
Dataset
KS-TmG.2.0 (Metagenome, KS)

Please cite as : Anderson L.N., R. Wu, W.C. Nelson, J.E. McDermott, K.S. Hofmockel, and J.K. Jansson. 2021. KS-TmG.2.0 (Metagenome, KS). [Data Set] PNNL DataHub. https://doi.org/10.25584/KSTmG2/1770332 Soil samples were collected in triplicate in the fall of 2017 across the three grassland locations...

Biology
Earth System Science
Dataset
WA-IsoC_MSC1.1.0 (Amplicon 16S rRNA, WA)

Please cite as : Anderson L.N., J.E. McDermott, and R.S. McClure. 2020. WA-IsoC_MSC1.1.0 (Amplicon 16S rRNA, WA). [Data Set] PNNL DataHub. https://doi.org/10.25584/WAIsoCMSC1/1635272 The soil microbiome is central to the cycling of carbon and other nutrients and to the promotion of plant growth...

Biology
Earth System Science
Dataset
KS-TmG.1.0 (Metagenome, KS)
Please cite as : Anderson L.N., W.C. Nelson, J.E. McDermott, R. Wu, S.J. Fansler, Y. Farris, and J.K. Jansson, et al. 2020. KS-TmG.1.0 (Metagenome, KS). [Data Set] PNNL DataHub. https://doi.org/10.25584/KSTmG1/1635004 To enable a comprehensive survey of the metabolic potential of complex soil...
Biology
Earth System Science
Dataset
IA-TmG.1.0 (Metagenome, IA)
Please cite as : Anderson L.N., W.C. Nelson, J.E. McDermott, R. Wu, S.J. Fansler, Y. Farris, and J.K. Jansson, et al. 2020. IA-TmG.1.0 (Metagenome, IA). [Data Set] PNNL DataHub. https://doi.org/10.25584/IATmG1/1635005 To enable a comprehensive survey of the metabolic potential of complex soil...
Biology
Earth System Science
Dataset
WA-TmG.1.0 (Metagenome, WA)

Please cite as : Anderson L.N., W.C. Nelson, J.E. McDermott, R. Wu, S.J. Fansler, Y. Farris, and J.K. Jansson, et al. 2020. WA-TmG.1.0 (Metagenome, WA). [Data Set] PNNL DataHub. https://doi.org/10.25584/WATmG1/1635002 To enable a comprehensive survey of the metabolic potential of complex soil...

Biology
Earth System Science
Dataset
WA-IsoC_NAG.1.0 (Amplicon 16S/ITS, WA)
Please cite as : Zegeye E., C.J. Brislawn, Y. Farris, S.J. Fansler, K.S. Hofmockel, J.K. Jansson, and A.T. Wright, et al. 2019. WA-IsoC_NAG.1.0 (Amplicon 16S/ITS, WA). [Data Set] PNNL DataHub. https://dx.doi.org/10.25584/data.2019-02.700/1506698 Investigation of the successional dynamics of a soil...
Biology
Earth System Science
Person
Caroline (Carrie) Harwood

Short Biography Caroline (Carrie) Harwood received her Ph.D. in microbiology from the University of Massachusetts and completed postdoctoral work at Yale University. She held academic appointments at Cornell University and the University of Iowa before moving to the University of Washington in 2005...

Biology
Microbiome Science
Plant Science
Publication
Draft Genome of Fusarium sp. strain DS 682, a novel Fungal isolate from the Grass Rhizosphere
The novel fungal strain, Fusarium sp. DS 682, was isolated from the rhizosphere of the perennial grass, Bouteloua gracilis , at the Konza Prairie Biological Station in Kansas. This fungal strain is common across North American grasslands and is resilient to environmental fluctuations. The draft...
Earth System Science
Biology
Publication
Development and Analysis of a Stable, Reduced Complexity Model Soil Microbiome
The soil microbiome is central to the cycling of carbon and other nutrients and to the promotion of plant growth. Despite its importance, analysis of the soil microbiome is difficult due to its sheer complexity, with thousands of interacting species. Here, we reduced this complexity by developing...
Biology
Earth System Science
Publication
Terabase Metagenome Sequencing of Grassland Soil Microbiomes
As part of the Pacific Northwest National Laboratory’s (PNNL) Science Focus Area program, we are investigating the impact of environmental change on microbial community function in grassland soils. Three grassland soils, representing different moisture regimes, were selected for ultra-deep...
Earth System Science
Biology
Publication
Selection, Succession, and Stabilization of Soil Microbial Consortia
Soil microorganisms play fundamental roles in cycling of soil carbon, nitrogen, and other nutrients, yet we have a poor understanding of how soil microbiomes are shaped by their nutritional and physical environment. In this study, we investigated the successional dynamics of a soil microbiome during...
Earth System Science
Biology