Fluorescently tagged bacterial soil isolates were cultured with N-acetylglucosamine, chitopentose, or chitin in porous SoilChip devices designed to mimic the structural habitats found in soil. Imaging analysis was used to examine how microbial traits, nutrient substrate solubility and degree of...

The data package consists of isolated pancreatic islets from adult male C57BL6/J mice treated with IL-1β, IFNγ or IL-1β + IFNγ for 6 h and submitted for scRNA-seq. This study focused on understanding the heterogeneity of the cytokine-mediated response. Data contributors: Jennifer S. Stancill & John...

The data package consists of RNA-seq datasets of EndoC-βH1 cells treated in parallel with IFNα, IFNγ, IL-1β and TNFα for 24 h and submitted to RNA-seq (n=4) ( Table 1 ) Data contributors: Arturo Roca-Rivada, Xiaoyan Yi & Décio L. Eizirik: ULB Center for Diabetes Research, Medical Faculty, Université...

This data package consists of RNA-seq data from human islets (n=10) treated with IFNγ + IL-1β for 24 h ( Table 1 ). Data contributors: Wenting Wu & Carmella Evans-Molina: Center for Diabetes and Metabolic Diseases, Indiana University School of Medicine, Indianapolis, IN, USA. Farooq Syed: Center for...

This data package consists of data from β-TC-6 cells treated with IFNγ + IL-1β for 4, 8 or 24 h (n=4) and submitted to enrichment of cysteine-containing peptides (redox), phosphopeptides and acetylated peptides. This enabled the analysis of proteomics, redox cysteine, phosphoproteomics and...

This data package consists of data from human islets treated for 48 h with cytokines (IFNγ, TNFα, and/or IL1β), thapsigargin (TG), brefeldin A (BFA), or DMSO for the control (CTRL) and submitted for single-cell RNA-seq (n=5) and single-nucleus multi-omics sequencing (snMultiomics-seq, n=1) ( Table 1...

The data package consists of RNA-seq data from human islet-derived exosomes post IFNγ + IL-1β treatment of human islets (n=5) for 24 h ( Table 1 ). Data contributors: Preethi Krishnan& Carmella Evans-Molina: Department of Diabetes Immunology, Beckman Research Institute, City of Hope, Duarte, CA, USA...

This data package consists of data from human islets and EndoC-βH1 cells treated with IFNα for 2, 8, or 24 h and submitted to RNA-seq (human islets: n=6 & EndoC-βH1: n=5) and ATAC-seq (n=4). In parallel, a batch of cells (n=4) had proteomics, lipidomics, and metabolomics performed in the same...

This data package consists of data from MIN6 cells (n=4) treated with IL-1β + IFNγ + TNFα for 24 h post RNAi targeting 85/88 kDa calcium-independent phospholipase A2 ( Pla2g6 ) and submitted to proteomics analysis ( Table 1 ). Pla2g6 is an enzyme that metabolizes phosphatidylcholine (PCs) into...

This data package consists of data from MIN6 cells (n=4) treated with IL-1β + IFNγ + TNFα for 24 h post-RNAi targeting ADP-ribosylhydrolase ARH3 (Adprhl2), and submitted to proteomics analysis ( Table 1 ). ARH3 is a hydrolase that keeps cellular ADP-ribosylation levels in check and is regulated by...

This data package consists of data from MIN6 cells (n=3) treated with IFNγ + IL-1β + TNFα with or without DFMO or N1,N11-diethylnorspermine (DENSpm) and submitted to RNA-seq ( Table 1 ). DENSpm is an inhibitor of ornithine decarboxylase (ODC) and polyamine oxidase (PAOX), both enzymes in the...

The data package consists of data from iPSC: HEL115.6-derived islet-like aggregates treated with IFNα for 24 h and submitted to RNA-seq (n=5) and scRNA-seq (n=5). ( Table 1 ). Data contributors: Arturo Roca-Rivada, Xiaoyan Yi & Décio L. Eizirik: ULB Center for Diabetes Research, Medical Faculty...

This data package consists data from EndoC-βH1 (n=6) treated with IFNα for 8 and 24 h and submitted to RNA-seq analysis ( Table 1 ). This was generated in parallel with Pck008. Data contributors: Arturo Roca-Rivada, Xiaoyan Yi & Décio L. Eizirik: ULB Center for Diabetes Research, Medical Faculty...

This data package consists of data from human islets (n=5) treated with IFNγ + IL-1β for 48 h and submitted to RNA-seq analysis ( Table 1 ). Data contributors: Arturo Roca-Rivada, Xiaoyan Yi & Décio L. Eizirik: ULB Center for Diabetes Research, Medical Faculty, Université Libre de Bruxelles...

This data package consists of data from MIN6 cells (n=4) treated with IL-1β + IFNγ + TNFα and RNAi targeting Poly(ADP)ribosyl polymerase 12 ( Parp12 ) for 24 h and submitted to proteomic analyses ( Table 1 ). PARP12 is an interferon-induced protein known to target viral RNA to stress granules. It...