Biography Ernesto Nakayasu is a senior research scientist focused on understanding molecular mechanisms of diseases. Nakayasu has been applying systems biology and mass spectrometry-based omics measurements to study how pathogens and metabolic alterations cause human diseases; the goal is to...
The Thermo Scientific LTQ Orbitrap Velos combines the proven mass accuracy and ultra-high resolution of the Orbitrap mass analyzer, with the increased sensitivity and improved cycle time of the LTQ Velos. The high mass accuracy of the LTQ Orbitrap Velos increases the speed and condense of indication...
The IONTOF TOF.SIMS 5 data source is a time-of-flight secondary ion mass spectrometer and powerful surface analysis tool used to investigate scientific questions in biological, environmental, and energy research. Among the most sensitive of surface analysis tools, it uses a high-vacuum technique...
LIQUID Software Overview LIQUID provides users with the capability to process high throughput data and contains a customizable target library and scoring model per project needs. The graphical user interface provides visualization of multiple lines of spectral evidence for each lipid identification...
Model Soil Consortium 2 (MSC-2) Bacterial Isolate Genomes Model Soil Consortium 2 (MSC-2) bacterial isolate genome collections are derived from the soil consortium "MSC-1" multi-species cultured isolate genome collections from a previously reported WA-IsoC_MSC1.1.0 collection of a naturally evolved...
PerCon SFA Project Publication Linked Data Catalog (uploads pending) The Persistence Control of Engineered Functions in Complex Soil Microbiomes Project (PerCon SFA), at Pacific Northwest National Laboratory (PNNL) is a Genomic Sciences Program, Biosystems Design, Research Science Focus Area project...
"Visualizing the Hidden Half: Plant-Microbe Interactions in the Rhizosphere" Plant roots and the associated rhizosphere constitute a dynamic environment that fosters numerous intra- and interkingdom interactions, including metabolite exchange between plants and soil mediated by root exudates and the...
Pending Review Microbiomes contribute to multiple ecosystem services by transforming organic matter in soil. Extreme shifts in the environment, such as drying-rewetting cycles during drought, can impact microbial metabolism of organic matter by altering their physiology and function. These...
Multi-Omics Extended Data Package DOI Rapid Remodeling of the Soil Lipidome in Response to a Drying-Rewetting Event This study reveals specific changes in lipids and metabolites that are indicative of stress adaptation, substrate use, and cellular recovery during soil drying and subsequent rewetting...
MPLEx Method Protocol for Integrative Single-Sample Processing for Subsequent Proteomic, Metabolomic, and Lipidomic Analyses The Metabolite, Protein and Lipid Extraction (MPLEx) method protocol is a robust sample processing technique applicable to a breadth of diverse sample types, including various...
"DNA Viral Diversity, Abundance, and Functional Potential Vary across Grassland Soils with a Range of Historical Moisture Regimes" Soil viruses are abundant, but the influence of the environment and climate on soil viruses remains poorly understood. Here, we addressed this gap by comparing the...
"Deconstructing the Soil Microbiome into Reduced-Complexity Functional Modules" The soil microbiome represents one of the most complex microbial communities on the planet, encompassing thousands of taxa and metabolic pathways, rendering holistic analyses computationally intensive and difficult. Here...
Soil samples were collected in triplicate in the fall of 2017 across the three grassland locations having differences in historical annual precipitation field site locations (WA, IA, KS). For each location, one deeply sequenced composite metagenome was obtained to detect viral contigs using a...
Soil samples were collected in triplicate in the fall of 2017 across the three grassland locations having differences in historical annual precipitation field site locations (WA, IA, KS). For each location, one deeply sequenced composite metagenome was obtained to detect viral contigs using a...
Soil samples were collected in triplicate in the fall of 2017 across the three grassland locations having differences in historical annual precipitation field site locations (WA, IA, KS). For each location, one deeply sequenced composite metagenome was obtained to detect viral contigs using a...