Illumina MiSeq System Sequencer


The Illumina MiSeq System Sequencer is a high-throughput DNA sequencer machine developed and manufactured by Illumina, and is designed for sequencing data acquisition using synthesis technology to provide an end-to-end solution (cluster generation, amplification, sequencing, and data analysis) in a single machine. Primary applications include: small whole-genome sequencing, targeted sequencing of a set of genes or gene regions and 16S metagenomics sequencing. 

MiSeq pre-processed raw data is packaged in a .fastq format and can be accessed at project dataset download DOI containing additional processed data products, metadata, and software workflows related to a specific project sequence analysis dataset collection (or catalog) using deoxyribonucleic acid DNA sequencing products.

Data Source Contributions

Instrument Data Source Profile

Source Method DOI:       Pending

Instrument Platform:      Illumina MiSeq System is a data collection device

Source Taxonomy:           Measurement DeviceInstrument > Sequencer > High Throughput Sequencer

                                                > Illumina MiSeq System Sequencer (EFO:0004205) sameAs MiSeq

Data Type:                         Biological DataGenetic data Transcriptomic data

Data Format:                     Data itemDatasetFASTQ format > Illumina native fastq format

Method Type:                    Molecular Biology Methodnucleic acid identification and quantitation method

Laboratory Technique:     Measurement Technique using a ProcedureStandard Operating Procedure

                                                > Nucleic acid sequencing procedure > Analysis using pyrosequencing technique procedure

Experimental Design:       Assay > Experimental Protocol > nucleic acid sequencing protocol

                                                > Illumina MiSeq standard manufacturer's protocol

Research Topic Areas:     Scientific DataTranscriptomics, SequencingPyrosequencing

Research Categories:      Soil MicrobiomeIntegrative OmicsMicrobiome ScienceBiology



    This work was supported in part by the Earth and Biological Sciences Directorate (EBSD) at Pacific Northwest National Laboratory (PNNL), a multiprogram national laboratory managed by the Battelle Memorial Institute, operating under the U.S. Department of Energy (DOE) contract DE-AC05-76RL01830. User capabilities described here reflect collaborations with the Environmental Molecular Sciences Laboratory (EMSL), a DOE Office of Science (SC-3) user facility operating under the Contract No. DE-AC05-76RL01830.

    Projects (3)

    The influence of tidal inundation dynamics on below ground carbon pools is poorly understood across coastal terrestrial-aquatic interface (TAI) ecosystems. The dynamic environmental conditions of tidally-influenced landscapes, the chemically complex nature of carbon compounds, the diverse nature of...

    1. Datasets


    The Phenotypic Response of the Soil Microbiome to Environmental Perturbations Project (Soil Microbiome SFA) at Pacific Northwest National Laboratory is a Genomic Sciences Program Science Focus Area (SFA) Project operating under the Environmental Microbiome Science Research Area. The Soil Microbiome...

    1. Datasets


    PerCon SFA Project Publication Linked Data Catalog (uploads pending) The Persistence Control of Engineered Functions in Complex Soil Microbiomes Project (PerCon SFA), at Pacific Northwest National Laboratory (PNNL) is a Genomic Sciences Program, Biosystems Design, Research Science Focus Area project...

    1. Datasets

    Datasets (3)
    Publications (3)
    Ryan McClure
    Dan Naylor
    Yuliya Farris
    Michelle R Davison
    Sarah J. Fansler
    Kirsten Hofmockel
    Janet K. Jansson
    Dan Naylor
    Sarah J. Fansler
    Dr William C Nelson
    Kirsten Hofmockel
    Janet K. Jansson
    Ryan McClure
    People (2)

    Lindsey Anderson’s research has been dedicated to the identification and characterization of novel, targeted and non-targeted, functional metabolic interactions using a high-throughput systems biology and computational biology approach. Her expertise in functional metabolism and multidisciplinary...

    Publications: 2018 Stegen JC, TC Johnson, JK Fredrickson, MJ Wilkins, AE Konopka, WC Nelson, EV Arntzen, WB Chrisler, RK Chu, SJ Fansler, EB Graham, DW Kennedy, CT Resch, MM Tfaily, and JM Zachara. 2018. "Influences of organic carbon speciation on hyporheic corridor biogeochemistry and microbial...