Data Source - Instrument Illumina MiSeq System Sequencer

The Illumina MiSeq System Sequencer is a high-throughput DNA sequencer machine developed and manufactured by Illumina, and is designed for sequencing data acquisition using synthesis technology to provide an end-to-end solution (cluster generation, amplification, sequencing, and data analysis) in a single machine. Primary applications include: small whole-genome sequencing, targeted sequencing of a set of genes or gene regions and 16S metagenomics sequencing. 

MiSeq pre-processed raw data is packaged in a .fastq format and can be accessed at project dataset download DOI containing additional processed data products, metadata, and software workflows related to a specific project sequence analysis dataset collection (or catalog) using deoxyribonucleic acid DNA sequencing products.

Data Source Contributions

• LN Anderson (Domain Biocuration): Data curation, Writing – original draft, Writing – review & editing
• SJ Fansler (Expert Review): Resources, Validation

Instrument Data Source Profile

Source Method DOI:       Pending

Instrument Platform:      Illumina MiSeq System is a data collection device

Source Taxonomy:           Measurement Device, Instrument > Sequencer > High Throughput Sequencer

                                                > Illumina MiSeq System Sequencer (EFO:0004205) sameAs MiSeq

Data Type:                         Biological Data > Genetic data > Transcriptomic data

Data Format:                     Data item > Dataset, FASTQ format > Illumina native fastq format

Method Type:                    Molecular Biology Method > nucleic acid identification and quantitation method

Laboratory Technique:     Measurement Technique using a Procedure > Standard Operating Procedure

                                                > Nucleic acid sequencing procedure > Analysis using pyrosequencing technique procedure

Experimental Design:       Assay > Experimental Protocol > nucleic acid sequencing protocol

                                                > Illumina MiSeq standard manufacturer's protocol

Research Topic Areas:     Scientific Data, Transcriptomics, Sequencing > Pyrosequencing

Research Categories:      Soil Microbiome, Integrative Omics, Microbiome Science, Biology

Acknowledgements

This work was supported in part by the Earth and Biological Sciences Directorate (EBSD) at Pacific Northwest National Laboratory (PNNL), a multiprogram national laboratory managed by the Battelle Memorial Institute, operating under the U.S. Department of Energy (DOE) contract DE-AC05-76RL01830. User capabilities described here reflect collaborations with the Environmental Molecular Sciences Laboratory (EMSL), a DOE Office of Science (SC-3) user facility operating under the Contract No. DE-AC05-76RL01830.