Description
The Illumina MiSeq System Sequencer is a high-throughput DNA sequencer machine developed and manufactured by Illumina, and is designed for sequencing data acquisition using synthesis technology to provide an end-to-end solution (cluster generation, amplification, sequencing, and data analysis) in a single machine. Primary applications include: small whole-genome sequencing, targeted sequencing of a set of genes or gene regions and 16S metagenomics sequencing.
MiSeq pre-processed raw data is packaged in a .fastq format and can be accessed at project dataset download DOI containing additional processed data products, metadata, and software workflows related to a specific project sequence analysis dataset collection (or catalog) using deoxyribonucleic acid DNA sequencing products.
Data Source Contributions
Instrument Data Source Profile
Source Method DOI: Pending
Instrument Platform: Illumina MiSeq System is a data collection device
Source Taxonomy: Measurement Device, Instrument > Sequencer > High Throughput Sequencer
> Illumina MiSeq System Sequencer (EFO:0004205) sameAs MiSeq
Data Type: Biological Data > Genetic data > Transcriptomic data
Data Format: Data item > Dataset, FASTQ format > Illumina native fastq format
Method Type: Molecular Biology Method > nucleic acid identification and quantitation method
Laboratory Technique: Measurement Technique using a Procedure > Standard Operating Procedure
> Nucleic acid sequencing procedure > Analysis using pyrosequencing technique procedure
Experimental Design: Assay > Experimental Protocol > nucleic acid sequencing protocol
> Illumina MiSeq standard manufacturer's protocol
Research Topic Areas: Scientific Data, Transcriptomics, Sequencing > Pyrosequencing
Acknowledgements
This work was supported in part by the Earth and Biological Sciences Directorate (EBSD) at Pacific Northwest National Laboratory (PNNL), a multiprogram national laboratory managed by the Battelle Memorial Institute, operating under the U.S. Department of Energy (DOE) contract DE-AC05-76RL01830. User capabilities described here reflect collaborations with the Environmental Molecular Sciences Laboratory (EMSL), a DOE Office of Science (SC-3) user facility operating under the Contract No. DE-AC05-76RL01830.
Projects (3)
The influence of tidal inundation dynamics on below ground carbon pools is poorly understood across coastal terrestrial-aquatic interface (TAI) ecosystems. The dynamic environmental conditions of tidally-influenced landscapes, the chemically complex nature of carbon compounds, the diverse nature of...
Category
Datasets
3
The Phenotypic Response of the Soil Microbiome to Environmental Perturbations Project (Soil Microbiome SFA) at Pacific Northwest National Laboratory is a Genomic Sciences Program Science Focus Area (SFA) Project operating under the Environmental Microbiome Science Research Area. The Soil Microbiome...
Datasets
24
Last updated on 2023-02-23T19:37:46+00:00 by LN Anderson PerCon SFA Project Publication Experimental Data Catalog The Persistence Control of Engineered Functions in Complex Soil Microbiomes Project (PerCon SFA) at Pacific Northwest National Laboratory ( PNNL ) is a Genomic Sciences Program...
Datasets
3