Description
The Illumina MiSeq System Sequencer is a high-throughput DNA sequencer machine developed and manufactured by Illumina, and is designed for sequencing data acquisition using synthesis technology to provide an end-to-end solution (cluster generation, amplification, sequencing, and data analysis) in a single machine. Primary applications include: small whole-genome sequencing, targeted sequencing of a set of genes or gene regions and 16S metagenomics sequencing.
MiSeq pre-processed raw data is packaged in a .fastq format and can be accessed at project dataset download DOI containing additional processed data products, metadata, and software workflows related to a specific project sequence analysis dataset collection (or catalog) using deoxyribonucleic acid DNA sequencing products.
Data Source Contributions
- LN Anderson (Domain Biocuration): Data curation, Writing – original draft, Writing – review & editing
- SJ Fansler (Expert Review): Resources, Validation
Instrument Data Source Profile
Source Method DOI: Pending
Instrument Platform: Illumina MiSeq System is a data collection device
Source Taxonomy: Measurement Device, Instrument > Sequencer > High Throughput Sequencer
> Illumina MiSeq System Sequencer (EFO:0004205) sameAs MiSeq
Data Type: Biological Data > Genetic data > Transcriptomic data
Data Format: Data item > Dataset, FASTQ format > Illumina native fastq format
Method Type: Molecular Biology Method > nucleic acid identification and quantitation method
Laboratory Technique: Measurement Technique using a Procedure > Standard Operating Procedure
> Nucleic acid sequencing procedure > Analysis using pyrosequencing technique procedure
Experimental Design: Assay > Experimental Protocol > nucleic acid sequencing protocol
> Illumina MiSeq standard manufacturer's protocol
Research Topic Areas: Scientific Data, Transcriptomics, Sequencing > Pyrosequencing
Research Categories: Soil Microbiome, Integrative Omics, Microbiome Science, Biology
Acknowledgements
This work was supported in part by the Earth and Biological Sciences Directorate (EBSD) at Pacific Northwest National Laboratory (PNNL), a multiprogram national laboratory managed by the Battelle Memorial Institute, operating under the U.S. Department of Energy (DOE) contract DE-AC05-76RL01830. User capabilities described here reflect collaborations with the Environmental Molecular Sciences Laboratory (EMSL), a DOE Office of Science (SC-3) user facility operating under the Contract No. DE-AC05-76RL01830.
Projects (3)
The influence of tidal inundation dynamics on below ground carbon pools is poorly understood across coastal terrestrial-aquatic interface (TAI) ecosystems. The dynamic environmental conditions of tidally-influenced landscapes, the chemically complex nature of carbon compounds, the diverse nature of...
Category
Datasets
3
The Phenotypic Response of the Soil Microbiome to Environmental Perturbations Project (Soil Microbiome SFA) at Pacific Northwest National Laboratory is a Genomic Sciences Program Science Focus Area (SFA) Project operating under the Environmental Microbiome Science Research Area. The Soil Microbiome...
Datasets
13
PerCon SFA Project Publication Linked Data Catalog (uploads pending) The Persistence Control of Engineered Functions in Complex Soil Microbiomes Project (PerCon SFA), at Pacific Northwest National Laboratory (PNNL) is a Genomic Sciences Program, Biosystems Design, Research Science Focus Area project...
Datasets
0