Soil microorganisms play fundamental roles in cycling of soil carbon, nitrogen, and other nutrients, yet we have a poor understanding of how soil microbiomes are shaped by their nutritional and physical environment. In this study, we investigated the successional dynamics of a soil microbiome during...
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As part of the Pacific Northwest National Laboratory’s (PNNL) Science Focus Area program, we are investigating the impact of environmental change on microbial community function in grassland soils. Three grassland soils, representing different moisture regimes, were selected for ultra-deep...
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The soil microbiome is central to the cycling of carbon and other nutrients and to the promotion of plant growth. Despite its importance, analysis of the soil microbiome is difficult due to its sheer complexity, with thousands of interacting species. Here, we reduced this complexity by developing...
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"Deconstructing the Soil Microbiome into Reduced-Complexity Functional Modules" The soil microbiome represents one of the most complex microbial communities on the planet, encompassing thousands of taxa and metabolic pathways, rendering holistic analyses computationally intensive and difficult. Here...
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The rhizosphere represents a dynamic and complex interface between plant hosts and the microbial community found in the surrounding soil. While it is recognized that manipulating the rhizosphere has the potential to improve plant fitness and health, engineering the rhizosphere microbiome through...
Agriculture is the largest source of greenhouse gases (GHG) production. Conversion of nitrogen fertilizers into more reduced forms by microbes through a process known as biological nitrification drives GHG production, enhances proliferation of toxic algal blooms, and increases cost of crop...
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A major challenge in biotechnology and biomanufacturing is the identification of a set of biomarkers for perturbations and metabolites of interest. Here, we develop a data-driven, transcriptome-wide approach to rank perturbation-inducible genes from time-series RNA sequencing data for the discovery...
Last updated on 2023-02-23T19:37:46+00:00 by LN Anderson PerCon SFA Project Publication Experimental Data Catalog The Persistence Control of Engineered Functions in Complex Soil Microbiomes Project (PerCon SFA) at Pacific Northwest National Laboratory ( PNNL ) is a Genomic Sciences Program...
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Dr. Joshua Elmore is a Chemical Engineer at Pacific Northwest National Laboratory. He received his Ph. D. from the University of Georgia in the Department of Molecular Biology and Biochemistry studying the mechanisms of CRISPR/Cas microbial immune systems in hyperthermophilic Archaea. He was a...
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Elise Van Fossen is a Post-Doctorate Research Associate in the Biological Sciences Division. Her research focuses on developing synthetic and chemical biology techniques for microorganism engineering.
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Please cite as : Anderson L.N., W.C. Nelson, J.E. McDermott, R. Wu, S.J. Fansler, Y. Farris, and J.K. Jansson, et al. 2020. WA-TmG.1.0 (Metagenome, WA). [Data Set] PNNL DataHub. https://doi.org/10.25584/WATmG1/1635002 To enable a comprehensive survey of the metabolic potential of complex soil...
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Please cite as : Anderson L.N., W.C. Nelson, J.E. McDermott, R. Wu, S.J. Fansler, Y. Farris, and J.K. Jansson, et al. 2020. IA-TmG.1.0 (Metagenome, IA). [Data Set] PNNL DataHub. https://doi.org/10.25584/IATmG1/1635005 To enable a comprehensive survey of the metabolic potential of complex soil...
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Please cite as : Anderson L.N., W.C. Nelson, J.E. McDermott, R. Wu, S.J. Fansler, Y. Farris, and J.K. Jansson, et al. 2020. KS-TmG.1.0 (Metagenome, KS). [Data Set] PNNL DataHub. https://doi.org/10.25584/KSTmG1/1635004 To enable a comprehensive survey of the metabolic potential of complex soil...
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Please cite as : Anderson L.N., J.E. McDermott, and R.S. McClure. 2020. WA-IsoC_MSC1.1.0 (Amplicon 16S rRNA, WA). [Data Set] PNNL DataHub. https://doi.org/10.25584/WAIsoCMSC1/1635272 The soil microbiome is central to the cycling of carbon and other nutrients and to the promotion of plant growth...
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Last updated on 2023-03-21T18:35:22+00:00 by LN Anderson SAGE-RTP RT-PCR Amplicon Sequencing Barcode Count Analysis Promoter expression data for five bacterial species associated with the Serine recombinase Assisted Genome Engineering (SAGE) research project. Raw Measurement Data NCBI BioProject...
