Illumina HiSeq X Sequencing System

Description

The Illumina HiSeq X System Sequencer is a high-throughput DNA sequencer machine developed and manufactured by Illumina, and is designed for high throughput, production-scale sequencing laboratories. Built off the HiSeq 2500 System, harnessing the patterned flow cell technology originally developed for the HiSeq X™ Ten System, the Illumina HiSeq X platforms provide high-throughput sequencing offering project design flexibility and scalability for a wide variety of project investigations. Services used for this sequencing platform were contracted through GeneWiz (NGS platform resources). Primary applications include population-scale whole genome WGS sequencing and a variety of other services geared towards both biomedical and environmental project applications.

HiSeq X pre-processed raw data is packaged in a .fastq format and can be accessed at project dataset download DOI containing additional processed data products, metadata, and software related to a sequence analysis using deoxyribonucleic acid DNA sequencing products.

Data Source Contributions

Instrument Data Source Profile

Instrument Platform:       Illumina HiSeq X System, a data collection device

Source Taxonomy:           Measurement DeviceInstrument > Sequencer > High Throughput Sequencer

                                                > Illumina HiSeq X System Sequencer (EFO:0008567) isPartOf HiSeq X Ten

Data Type:                         Biological Data > Genetic data Genomic data

Data Format:                     Data item > DatasetFASTQ format > Illumina native fastq format

Method Type:                    Molecular Biology Method > nucleic acid identification and quantitation method 

Laboratory Technique:     Measurement Technique using a Procedure > Standard Operating Procedure

                                                > Nucleic acid sequencing procedure > Whole genome sequencing procedure

Experimental Design:       Assay > Experimental Protocol > nucleic acid sequencing protocol

                                                > Illumina HiSeq 2000 standard manufacturer's protocol

Research Topic Areas:     Scientific DataGenomicsSequencing > whole genome sequencing

Research Categories:       Soil MicrobiomeIntegrative OmicsMicrobiome ScienceBiology

 

Acknowledgements

This work was supported in part by the Earth and Biological Sciences Directorate (EBSD) at Pacific Northwest National Laboratory (PNNL), a multiprogram national laboratory managed by the Battelle Memorial Institute, operating under the U.S. Department of Energy (DOE) contract DE-AC05-76RL01830. User capabilities described here reflect collaborations with the Environmental Molecular Sciences Laboratory (EMSL), a DOE Office of Science (SC-3) user facility operating under the Contract No. DE-AC05-76RL01830.

 

*See attached technical document

Projects (2)

The Phenotypic Response of the Soil Microbiome to Environmental Perturbations Project (Soil Microbiome) at Pacific Northwest National Laboratory is a Genomic Sciences Program Science Focus Area (SFA) Project operating under the Environmental Microbiome Science Research Area. The Soil Microbiome SFA...

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PerCon SFA Project Data Catalog Collection (uploads pending) The Persistence Control of Engineered Functions in Complex Soil Microbiomes Project (PerCon SFA), at Pacific Northwest National Laboratory (PNNL), is a Genomic Sciences Program Biosystems Design Research Science Focus Area project. The...

  1. Datasets

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Datasets (8)
Publications (3)
Ruonan Wu
Michelle R Davison
Dr William C Nelson
Emily Graham
Sarah J. Fansler
Yuliya Farris
Jason McDermott
Kirsten Hofmockel
Janet K. Jansson
Sheryl L. Bell
Dr William C Nelson
Lindsey N. Anderson
Janet K. Jansson
Ruonan Wu
Jason McDermott
Sheryl L. Bell
Ari Jumpponen
Sarah J. Fansler
Kimberly J. Tyrrell
Yuliya Farris
Kirsten Hofmockel
Arunima Bhattacharjee
Lindsey N. Anderson
Andrea Porras-Alfaro
Ari Jumpponen
Kirsten Hofmockel
Janet K. Jansson
Dr Christopher R Anderton
Dr William C Nelson
People (2)

Lindsey Anderson’s research has been dedicated to the identification and characterization of novel, targeted and non-targeted, functional metabolic interactions using a high-throughput systems biology and computational biology approach. Her expertise in functional metabolism and multidisciplinary...

Publications: 2018 Stegen JC, TC Johnson, JK Fredrickson, MJ Wilkins, AE Konopka, WC Nelson, EV Arntzen, WB Chrisler, RK Chu, SJ Fansler, EB Graham, DW Kennedy, CT Resch, MM Tfaily, and JM Zachara. 2018. "Influences of organic carbon speciation on hyporheic corridor biogeochemistry and microbial...