Data Source - Sequencing Illumina HiSeq X Sequencing System

The Illumina HiSeq X System Sequencer is a high-throughput DNA sequencer machine developed and manufactured by Illumina, and is designed for high throughput, production-scale sequencing laboratories. Built off the HiSeq 2500 System, harnessing the patterned flow cell technology originally developed for the HiSeq X™ Ten System, the Illumina HiSeq X platforms provide high-throughput sequencing offering project design flexibility and scalability for a wide variety of project investigations. Services used for this sequencing platform were contracted through GeneWiz (NGS platform resources). Primary applications include population-scale whole genome WGS sequencing and a variety of other services geared towards both biomedical and environmental project applications.

HiSeq X pre-processed raw data is packaged in a .fastq format and can be accessed at project dataset download DOI containing additional processed data products, metadata, and software related to a sequence analysis using deoxyribonucleic acid DNA sequencing products.

Data Source Contributions

• LN Anderson > Data Curation
• SJ Fansler > Resource Review

Instrument Data Source Profile

Instrument Platform:       Illumina HiSeq X System, a data collection device

Source Taxonomy:           Measurement Device, Instrument > Sequencer > High Throughput Sequencer

                                                > Illumina HiSeq X System Sequencer (EFO:0008567) isPartOf HiSeq X Ten

Data Type:                         Biological Data > Genetic data > Genomic data

Data Format:                     Data item > Dataset, FASTQ format > Illumina native fastq format

Method Type:                    Molecular Biology Method > nucleic acid identification and quantitation method 

Laboratory Technique:     Measurement Technique using a Procedure > Standard Operating Procedure

                                                > Nucleic acid sequencing procedure > Whole genome sequencing procedure

Experimental Design:       Assay > Experimental Protocol > nucleic acid sequencing protocol

                                                > Illumina HiSeq 2000 standard manufacturer's protocol

Research Topic Areas:     Scientific Data, Genomics, Sequencing > whole genome sequencing

Research Categories:       Soil Microbiome, Integrative Omics, Microbiome Science, Biology

Acknowledgements

This work was supported in part by the Earth and Biological Sciences Directorate (EBSD) at Pacific Northwest National Laboratory (PNNL), a multiprogram national laboratory managed by the Battelle Memorial Institute, operating under the U.S. Department of Energy (DOE) contract DE-AC05-76RL01830. User capabilities described here reflect collaborations with the Environmental Molecular Sciences Laboratory (EMSL), a DOE Office of Science (SC-3) user facility operating under the Contract No. DE-AC05-76RL01830.