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Soil microorganisms play fundamental roles in cycling of soil carbon, nitrogen, and other nutrients, yet we have a poor understanding of how soil microbiomes are shaped by their nutritional and physical environment. In this study, we investigated the successional dynamics of a soil microbiome during...
The novel fungal strain, Fusarium sp. DS 682, was isolated from the rhizosphere of the perennial grass, Bouteloua gracilis , at the Konza Prairie Biological Station in Kansas. This fungal strain is common across North American grasslands and is resilient to environmental fluctuations. The draft...
As part of the Pacific Northwest National Laboratory’s (PNNL) Science Focus Area program, we are investigating the impact of environmental change on microbial community function in grassland soils. Three grassland soils, representing different moisture regimes, were selected for ultra-deep...
The soil microbiome is central to the cycling of carbon and other nutrients and to the promotion of plant growth. Despite its importance, analysis of the soil microbiome is difficult due to its sheer complexity, with thousands of interacting species. Here, we reduced this complexity by developing...

Viral communities detected from three large grassland soil metagenomes with historically different precipitation moisture regimes.

The Sequel II System Sequencer is a high-throughput DNA sequencer machine developed and manufactured by PacBio , and is designed for high throughput, production-scale sequencing laboratories. Originally released in 2015, the Sequel system provides Single Molecule, Real-Time (SMRT) sequencing core...

Last updated on 2024-02-11T22:41:43+00:00 by LN Anderson MERS-CoV Experiment MHAE001 The purpose of this experiment was to evaluate the human host response to wild-type MERS-CoV (icMERS-CoV) virus infection. Sample data was obtained from primary human airway epithelial (HAE) cells for mRNA, miRNA...

Last updated on 2024-02-11T22:41:43+00:00 by LN Anderson MERS-CoV Experiment MHAE002 The purpose of this experiment was to evaluate the human host response to wild-type MERS-CoV (strain EMC-2012) virus infection. Sample data was obtained from primary human microvascular endothelial cells (HMVE) for...

Last updated on 2024-02-11T22:41:43+00:00 by LN Anderson MERS-CoV Experiment MHAE003 The purpose of this experiment was to evaluate the human host response to wild-type MERS-CoV (strain EMC-2012) infection. Sample data was obtained from primary human airway epithelial cells (HAE) for mRNA...

Last updated on 2024-02-11T22:41:43+00:00 by LN Anderson MERS-CoV Experiment MCL001 The purpose of this experiment was to evaluate the human host response to wild-type Middle Eastern Respiratory Syndrome coronavirus (MERS-CoV), and mutants icMERS-CoV-RFP, icMERS-CoV-dNSP16, icMERS-CoV-d4B, and...

Last updated on 2024-02-11T22:41:43+00:00 by LN Anderson MERS-CoV Experiment MCL002 The purpose of this experiment was to evaluate the human host response to wild-type Middle Eastern Respiratory Syndrome coronavirus (MERS-CoV) and mutants icMERS-RFP, icMERS-DNSP16, and icMERS-d4B virus infection...

Last updated on 2024-02-11T22:41:43+00:00 by LN Anderson MERS-CoV Experiment MFB001 The purpose of this experiment was to evaluate the human host response to wild-type MERS-CoV (icMERS-CoV) virus infection. Sample data was obtained from primary human fibroblasts and processed for mRNA, miRNA...

Last updated on 2024-02-11T22:41:43+00:00 by LN Anderson Interferon Experiment IFNFB001 The purpose of this experiment was to evaluate the human host cellular response to interferon alpha/beta (IFNα/β) or interferon gamma (IFNγ) treatment. Sample data was obtained from primary human lung fibroblast...

Last updated on 2024-02-11T22:41:43+00:00 by LN Anderson Interferon Experiment IFNMVE001 The purpose of this experiment was to evaluate the human host interferon-stimulated cellular response to interferon alpha/beta (IFNαβ) or gamma (INFγ) treatment. Sample data was obtained from primary lung...

Last updated on 2024-02-11T22:41:43+00:00 by LN Anderson Interferon Experiment IFNaHUH001 The purpose of this experiment was to evaluate the human host cellular response to treatment with and without interferon alpha/beta (IFNα/β) treatment. Sample time course data was obtained from human hepatoma...