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Soil microorganisms play fundamental roles in cycling of soil carbon, nitrogen, and other nutrients, yet we have a poor understanding of how soil microbiomes are shaped by their nutritional and physical environment. In this study, we investigated the successional dynamics of a soil microbiome during...
As part of the Pacific Northwest National Laboratory’s (PNNL) Science Focus Area program, we are investigating the impact of environmental change on microbial community function in grassland soils. Three grassland soils, representing different moisture regimes, were selected for ultra-deep...
The soil microbiome is central to the cycling of carbon and other nutrients and to the promotion of plant growth. Despite its importance, analysis of the soil microbiome is difficult due to its sheer complexity, with thousands of interacting species. Here, we reduced this complexity by developing...

"Deconstructing the Soil Microbiome into Reduced-Complexity Functional Modules" The soil microbiome represents one of the most complex microbial communities on the planet, encompassing thousands of taxa and metabolic pathways, rendering holistic analyses computationally intensive and difficult. Here...

"Visualizing the Hidden Half: Plant-Microbe Interactions in the Rhizosphere" Plant roots and the associated rhizosphere constitute a dynamic environment that fosters numerous intra- and interkingdom interactions, including metabolite exchange between plants and soil mediated by root exudates and the...

Human infections caused by viral pathogens trigger a complex gamut of host responses that limit disease, resolve infection, generate immunity, and contribute to severe disease or death. Here, we present experimental methods and multi-omics data capture approaches representing the global host...

Please cite as : Anderson L.N., W.C. Nelson, J.E. McDermott, R. Wu, S.J. Fansler, Y. Farris, and J.K. Jansson, et al. 2020. IA-TmG.1.0 (Metagenome, IA). [Data Set] PNNL DataHub. https://doi.org/10.25584/IATmG1/1635005 To enable a comprehensive survey of the metabolic potential of complex soil...
Please cite as : Anderson L.N., W.C. Nelson, J.E. McDermott, R. Wu, S.J. Fansler, Y. Farris, and J.K. Jansson, et al. 2020. KS-TmG.1.0 (Metagenome, KS). [Data Set] PNNL DataHub. https://doi.org/10.25584/KSTmG1/1635004 To enable a comprehensive survey of the metabolic potential of complex soil...

Please cite as : Anderson L.N., W.C. Nelson, J.E. McDermott, R. Wu, S.J. Fansler, Y. Farris, and J.K. Jansson, et al. 2020. WA-TmG.1.0 (Metagenome, WA). [Data Set] PNNL DataHub. https://doi.org/10.25584/WATmG1/1635002 To enable a comprehensive survey of the metabolic potential of complex soil...

Last updated on 2023-05-02T18:08:23+00:00 by LN Anderson Fungal Monoisolate Multi-Omics Data Package DOI "KS4A-Omics1.0_FspDS68" Molecular mechanisms underlying fungal mineral weathering and nutrient translocation in low nutrient environments remain poorly resolved, due to the lack of a platform for...

Please cite as : Anderson L.N., J.E. McDermott, and R.S. McClure. 2020. WA-IsoC_MSC1.1.0 (Amplicon 16S rRNA, WA). [Data Set] PNNL DataHub. https://doi.org/10.25584/WAIsoCMSC1/1635272 The soil microbiome is central to the cycling of carbon and other nutrients and to the promotion of plant growth...

Rapid remodeling of the soil lipidome in response to a drying-rewetting event - Multi-Omics Data Package DOI Data package contents reported here are the first version and contain pre- and post-processed data acquisition and subsequent downstream analysis files using various data source instrument...

The Phenotypic Response of the Soil Microbiome to Environmental Perturbations Project (Soil Microbiome SFA) at Pacific Northwest National Laboratory is a Genomic Sciences Program Science Focus Area (SFA) Project operating under the Environmental Microbiome Science Research Area. The Soil Microbiome...

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LIQUID Software Overview LIQUID provides users with the capability to process high throughput data and contains a customizable target library and scoring model per project needs. The graphical user interface provides visualization of multiple lines of spectral evidence for each lipid identification...

pmartR Software Overview The pmartR package provides a single software tool for QC (filtering and normalization), exploratory data analysis (EDA), and statistical analysis (robust to missing data) and includes numerous visualization capabilities of mass spectrometry (MS) omics data (proteomic...