Soil microorganisms play fundamental roles in cycling of soil carbon, nitrogen, and other nutrients, yet we have a poor understanding of how soil microbiomes are shaped by their nutritional and physical environment. In this study, we investigated the successional dynamics of a soil microbiome during...
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The novel fungal strain, Fusarium sp. DS 682, was isolated from the rhizosphere of the perennial grass, Bouteloua gracilis , at the Konza Prairie Biological Station in Kansas. This fungal strain is common across North American grasslands and is resilient to environmental fluctuations. The draft...
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As part of the Pacific Northwest National Laboratory’s (PNNL) Science Focus Area program, we are investigating the impact of environmental change on microbial community function in grassland soils. Three grassland soils, representing different moisture regimes, were selected for ultra-deep...
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The soil microbiome is central to the cycling of carbon and other nutrients and to the promotion of plant growth. Despite its importance, analysis of the soil microbiome is difficult due to its sheer complexity, with thousands of interacting species. Here, we reduced this complexity by developing...
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To better understand the effects of solution chemistry on particle aggregation in the complex legacy tank wastes at the Hanford (WA) and Savannah River (SC) sites, we have performed a series of tumbler small- and ultra-small-angle neutron scattering experiments on 20 wt % solid slurries of...
Real-time electronic structure methods provide an unprecedented view of electron dynamics and ultrafast spectroscopy on the atto- and femtosecond time scale with vast potential to yield new insights into the electronic behavior of molecules and materials. In this Review, we discuss the fundamental...
The molecular speciation of aluminum (Al3+) in alkaline solutions is fundamental to its precipitation chemistry within a number of industrial applications that include ore refinement and industrial processing of Al wastes. Under these conditions, Al3+ is predominantly Al(OH)4–, while at high [Al3+]...
The quantum mechanical treatment of both electrons and nuclei is crucial in nonadiabatic dynamical processes such as proton-coupled electron transfer. The nuclear−electronic orbital (NEO) method provides an elegant framework for including nuclear quantum effects beyond the Born–Oppenheimer...
Last updated on 2024-02-11T22:41:43+00:00 by LN Anderson MERS-CoV Experiment MCL002 The purpose of this experiment was to evaluate the human host response to wild-type Middle Eastern Respiratory Syndrome coronavirus (MERS-CoV) and mutants icMERS-RFP, icMERS-DNSP16, and icMERS-d4B virus infection...
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Last updated on 2024-02-11T22:41:43+00:00 by LN Anderson MERS-CoV Experiment MFB001 The purpose of this experiment was to evaluate the human host response to wild-type MERS-CoV (icMERS-CoV) virus infection. Sample data was obtained from primary human fibroblasts and processed for mRNA, miRNA...
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Last updated on 2024-02-11T22:41:43+00:00 by LN Anderson MERS-CoV Experiment MFB002 The purpose of this experiment was to evaluate the human host response to wild-type MERS-CoV (icMERS-CoV) virus infection. Sample data was obtained from primary human fibroblasts for mRNA, proteomics, metabolomics...
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Last updated on 2024-02-11T22:41:43+00:00 by LN Anderson Influenza A Virus Experiment ICL103 The purpose of this experiment was to evaluate the human host response to Influenza A virus (subtype H5N1) and mutant viruses. Sample data was obtained for human lung adenocarcinoma cell line (Calu-3)...
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Last updated on 2024-02-11T22:41:43+00:00 by LN Anderson Influenza A Virus Experiment ICL104 The purpose of this experiment was to evaluate the host response to pandemic Influenza A virus (subtype H1N1) , natural isolate Influenza A/California/04/2009 virus. Sample data was obtained from human lung...
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Last updated on 2024-02-11T22:41:43+00:00 by LN Anderson Influenza A Virus Experiment ICL102 The purpose of this experiment was to evaluate the human host cellular response to Influenza A virus (subtype H7N9) , wild-type strain Influenza A/Anhui/1/2013 (AH1-WT), and mutant viruses NS1-L103F/I106M...
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Last updated on 2024-02-11T22:41:43+00:00 by LN Anderson MERS-CoV Experiment MM001 The purpose of this experiment was to evaluate the host response to wild-type MERS-CoV virus infection. Sample data was obtained from primary mouse (strain C57BL/6J) whole lung for mRNA, proteomics, metabolomics, and...
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