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As part of the Pacific Northwest National Laboratory’s (PNNL) Science Focus Area program, we are investigating the impact of environmental change on microbial community function in grassland soils. Three grassland soils, representing different moisture regimes, were selected for ultra-deep...
Dataset

Complete replicate terabase metagenome (TmG.2.0) of grassland soil microbiome collections from KPBS field site in Manhattan, KS. Metagenome (unclassified soil sequencing) Data DOI Package, version 2.0.

Dataset

Complete replicate terabase metagenome (TmG.2.0) of grassland soil microbiome collections from COBS field site in Boone County, IA. Metagenome (unclassified soil sequencing) Data DOI Package, version 2.0.

Dataset

Complete replicate terabase metagenome (TmG.2.0) of grassland soil microbiome collections from IAREC field site in Prosser, WA. Metagenome (unclassified soil sequencing) Data DOI Package, version 2.0.

Viral communities detected from three large grassland soil metagenomes with historically different precipitation moisture regimes.

Soil fungi facilitate the translocation of inorganic nutrients from soil minerals to other microorganisms and plants. This ability is particularly advantageous in impoverished soils, because fungal mycelial networks can bridge otherwise spatially disconnected and inaccessible nutrient hotspots...

"DNA Viral Diversity, Abundance, and Functional Potential Vary across Grassland Soils with a Range of Historical Moisture Regimes" Soil viruses are abundant, but the influence of the environment and climate on soil viruses remains poorly understood. Here, we addressed this gap by comparing the...

"Deconstructing the Soil Microbiome into Reduced-Complexity Functional Modules" The soil microbiome represents one of the most complex microbial communities on the planet, encompassing thousands of taxa and metabolic pathways, rendering holistic analyses computationally intensive and difficult. Here...

"Visualizing the Hidden Half: Plant-Microbe Interactions in the Rhizosphere" Plant roots and the associated rhizosphere constitute a dynamic environment that fosters numerous intra- and interkingdom interactions, including metabolite exchange between plants and soil mediated by root exudates and the...

The Sequel II System Sequencer is a high-throughput DNA sequencer machine developed and manufactured by PacBio , and is designed for high throughput, production-scale sequencing laboratories. Originally released in 2015, the Sequel system provides Single Molecule, Real-Time (SMRT) sequencing core...

Last updated on 2024-02-11T22:41:43+00:00 by LN Anderson MERS-CoV Experiment MMVE002 The purpose of this experiment was to evaluate the human host response to MERS-CoV (strain EMC-2012) infectious clone (icMERS-CoV) virus infection. Sample data was obtained from primary human microvascular...

Last updated on 2024-02-11T22:41:43+00:00 by LN Anderson Ebola Virus Experiment EHUH001 The purpose of this experiment was to evaluate the human host response to wild-type Zaire Ebola virus (strain Mayinga) and mutant virus infection. Samples were obtained from human hepatoma carcinoma cells (HUH-7)...

Last updated on 2024-02-11T22:41:43+00:00 by LN Anderson Ebola Virus Experiment EHUH002 The purpose of this experiment was to evaluate the human host response to wild-type Zaire Ebola virus (strain Mayinga) and mutant virus infection. Samples were obtained from human hepatoma carcinoma cells (HUH-7)...

Last updated on 2024-02-11T22:41:43+00:00 by LN Anderson MERS-CoV Experiment MCL001 The purpose of this experiment was to evaluate the human host response to wild-type Middle Eastern Respiratory Syndrome coronavirus (MERS-CoV), and mutants icMERS-CoV-RFP, icMERS-CoV-dNSP16, icMERS-CoV-d4B, and...

Last updated on 2024-02-11T22:41:43+00:00 by LN Anderson MERS-CoV Experiment MCL002 The purpose of this experiment was to evaluate the human host response to wild-type Middle Eastern Respiratory Syndrome coronavirus (MERS-CoV) and mutants icMERS-RFP, icMERS-DNSP16, and icMERS-d4B virus infection...